Application | Comment | Organism |
---|---|---|
drug development | potential target or the development of new antibacterial agents | Plasmodium falciparum |
drug development | potential target or the development of new antibacterial agents | Escherichia coli |
drug development | potential target or the development of new antibacterial agents | Pseudomonas aeruginosa |
drug development | potential target or the development of new antibacterial agents | Staphylococcus aureus |
drug development | potential target or the development of new antibacterial agents | Streptococcus pneumoniae |
drug development | potential target or the development of new antibacterial agents | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli | Pseudomonas aeruginosa |
expressed as His-tag fusion protein in Escherichia coli | Staphylococcus aureus |
expressed as His-tag fusion protein in Escherichia coli | Streptococcus pneumoniae |
expressed as His-tag fusion protein in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method | Plasmodium falciparum |
sitting drop vapor diffusion method | Escherichia coli |
sitting drop vapor diffusion method | Pseudomonas aeruginosa |
sitting drop vapor diffusion method | Staphylococcus aureus |
sitting drop vapor diffusion method | Streptococcus pneumoniae |
sitting drop vapor diffusion method | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Plasmodium falciparum | |
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Escherichia coli | |
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Staphylococcus aureus | |
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Streptococcus pneumoniae | |
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Thermotoga maritima | |
Fe2+ | required, irreversible oxidation to Fe3+ results in almost complete loss of activity | Plasmodium falciparum | |
Fe2+ | required, irreversible oxidation to Fe3+ results in almost complete loss of activity | Escherichia coli | |
Fe2+ | required, irreversible oxidation to Fe3+ results in almost complete loss of activity | Staphylococcus aureus | |
Fe2+ | required, irreversible oxidation to Fe3+ results in almost complete loss of activity | Streptococcus pneumoniae | |
Fe2+ | required, irreversible oxidation to Fe3+ results in almost complete loss of activity | Thermotoga maritima | |
Ni2+ | can replace Zn2+ | Pseudomonas aeruginosa | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Plasmodium falciparum | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Escherichia coli | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Staphylococcus aureus | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Streptococcus pneumoniae | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Thermotoga maritima | |
Zn2+ | required | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-methionine-polypeptide + H2O | Plasmodium falciparum | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Escherichia coli | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Pseudomonas aeruginosa | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Staphylococcus aureus | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Streptococcus pneumoniae | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Thermotoga maritima | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Staphylococcus aureus ATCC 2913 | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6K3 | - |
- |
Plasmodium falciparum | - |
- |
- |
Pseudomonas aeruginosa | Q9I7A8 | strain ATCC27853 | - |
Staphylococcus aureus | P68826 | strain ATCC2913 | - |
Staphylococcus aureus ATCC 2913 | P68826 | strain ATCC2913 | - |
Streptococcus pneumoniae | Q9F2F0 | strain ATCC6305 | - |
Thermotoga maritima | P96113 | - |
- |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | P96113 | - |
- |
Oxidation Stability | Organism |
---|---|
instable towards oxidation due to the oxidation of the metal ligating cysteine residue | Plasmodium falciparum |
instable towards oxidation due to the oxidation of the metal ligating cysteine residue | Escherichia coli |
instable towards oxidation due to the oxidation of the metal ligating cysteine residue | Pseudomonas aeruginosa |
instable towards oxidation due to the oxidation of the metal ligating cysteine residue | Staphylococcus aureus |
instable towards oxidation due to the oxidation of the metal ligating cysteine residue | Streptococcus pneumoniae |
instable towards oxidation due to the oxidation of the metal ligating cysteine residue | Thermotoga maritima |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Pseudomonas aeruginosa |
recombinant enzyme using His-tag | Staphylococcus aureus |
recombinant enzyme using His-tag | Streptococcus pneumoniae |
recombinant enzyme using His-tag | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Plasmodium falciparum | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Escherichia coli | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Pseudomonas aeruginosa | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Staphylococcus aureus | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Streptococcus pneumoniae | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Thermotoga maritima | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Staphylococcus aureus ATCC 2913 | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | formate + L-methionine-polypeptide | - |
? |