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Literature summary for 3.5.1.88 extracted from

  • Rajagopalan, P.T.R.; Datta, A.; Pei, D.
    Purification, characterization, and inhibition of peptide deformylase from Escherichia coli (1997), Biochemistry, 36, 13910-13918.
    View publication on PubMed
Search for more literature for 3.5.1.88

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

General Stability

General Stability Organism
extremely labile Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline competitive Escherichia coli
1,2-Ethanedithiol irreversible Escherichia coli
1,3-propanedithiol irreversible Escherichia coli
1,5-pentanedithiol
-
 Escherichia coli
2,3-dimercapto-1-propanesulfonic acid
-
 Escherichia coli
2,3-dimercapto-1-propanol
-
 Escherichia coli
2-mercaptoethanol 30% inhibition at 1 mM Escherichia coli
EDTA slight inhibition at 10 mM Escherichia coli
glutathione 10% inhibition at 1 mM Escherichia coli
p-chloromercuribenzoate potent inhibitor, IC50: 0.07 mM Escherichia coli
Thiophenol IC50: 0.5 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 N-formylmethionyl-Leu-p-nitroanilide
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19200
-
 gel filtration, electrospray ionization mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formyl-L-methionine-polypeptide + H2O Escherichia coli requires at least a dipeptide as substrate, increasing activity with increasing peptide length until it reaches the tetrapeptide formate + L-methionine-polypeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, 33% glycerol, 2 years Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-L-Met-Leu-p-nitroanilide + H2O
-
 Escherichia coli formate + Met-Leu-p-nitroanilide
-
 ?
N-formyl-Ala-Gly-Ser-Glu + H2O poor activity, preference of N-terminal methionine Escherichia coli formate + Ala-Gly-Ser-Glu
-
 ?
N-formyl-His-Ala-Ser-Arg + H2O greatly attenuated activity, preference for N-terminal methionine Escherichia coli formate + His-Ala-Ser-Arg
-
 ?
N-formyl-L-Met-D-Ala-Ser-Arg + H2O strong requirement for L-methionine, but D-amino acids tolerated in other positions Escherichia coli formate + L-Met-D-Ala-Ser-Arg
-
 ?
N-formyl-L-methionine-polypeptide + H2O requires at least a dipeptide as substrate, increasing activity with increasing peptide length until it reaches the tetrapeptide Escherichia coli formate + L-methionine-polypeptide
-
 ?
N-formyl-L-Nle-L-Ala-L-Ser + H2O norleucine is the only excepted substitution for methionine Escherichia coli formate + L-Nle-L-Ala-L-Ser
-
 ?
N-formyl-Met + H2O poor activity Escherichia coli formate + Met
-
 ?
N-formyl-Met-Leu-p-nitroanilide + H2O best substrate Escherichia coli formate + Met-Leu-p-nitroanilide
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 19200, electrospray ionization mass spectrometry Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 rapidly inactivated within 18-22°C in 2 min Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 sharp decrease of activity below and above Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0029
-
 1,3-propanedithiol
-
 Escherichia coli
0.004
-
 1,2-Ethanedithiol
-
 Escherichia coli
0.0109
-
 2,3-dimercapto-1-propanol
-
 Escherichia coli
0.038
-
 1,5-pentanedithiol
-
 Escherichia coli
0.0402
-
 2,3-dimercapto-1-propanesulfonic acid
-
 Escherichia coli
0.17
-
 1,10-phenanthroline
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.07
-
 potent inhibitor, IC50: 0.07 mM Escherichia coli p-chloromercuribenzoate
0.5
-
 IC50: 0.5 mM Escherichia coli Thiophenol