Literature summary for 3.5.1.88 extracted from

Tran, H.T.; Pham, T.V.; Ngo, H.P.; Hong, M.K.; Ahn, Y.J.; Kang, L.W.
Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Campylobacter jejuni (2013), Acta Crystallogr. Sect. F, 69, 1120-1122.

Cloned(Commentary)

Cloned (Comment)	Organism
gene def, recombinant expression of the enzyme, containing an N-terminal His6-tag followed by a Tobacco etch virus protease cleavage site and three alanine residues before the NdeI site to facilitate purification of the expressed protein, in Escherichia coli strain BL21(DE3)	Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant detagged enzyme, hanging drop vapour diffusion method, mixing of 900 nl of 6 mg/ml protein in 20 mM TrisHCl pH 8.0, 20 mM NaCl, 3 mM 2-mercaptoethanol, with 900 nl reservoir solution containing 2 M zinc acetate dihydrate, 20% w/v PEG 3350, pH 6.4, and euilibration against 1 ml reservoir solution, microseeding and buffer optimization, 4 days, X-ray diffraction structure determination and analysis at 2.9 A resolution	Campylobacter jejuni

Crystallization (Comment)

Organism

purified recombinant detagged enzyme, hanging drop vapour diffusion method, mixing of 900 nl of 6 mg/ml protein in 20 mM TrisHCl pH 8.0, 20 mM NaCl, 3 mM 2-mercaptoethanol, with 900 nl reservoir solution containing 2 M zinc acetate dihydrate, 20% w/v PEG 3350, pH 6.4, and euilibration against 1 ml reservoir solution, microseeding and buffer optimization, 4 days, X-ray diffraction structure determination and analysis at 2.9 A resolution

Campylobacter jejuni

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	-	gene def	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, cation exchange chromatography and dialysis	Campylobacter jejuni

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)