Cloned (Comment) | Organism |
---|---|
into pET28c vector for over-expression in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C106S | inactive mutant | Mycobacterium tuberculosis |
DELTA182-197 | deletion mutant, amino acids 182-197 were removed from the carboxy-terminal end, inactive | Mycobacterium tuberculosis |
H148A | inactive mutant | Mycobacterium tuberculosis |
H152A | inactive mutant | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Mycobacterium tuberculosis | |
actinonin | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.1 | - |
N-formyl-L-Met-L-Ala | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Mycobacterium tuberculosis | |
Zn2+ | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE, Western blotting using an anti-his tag antibody | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-L-methionyl peptide + H2O | Mycobacterium tuberculosis | - |
formate + methionyl peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA column | Mycobacterium tuberculosis |
Renatured (Comment) | Organism |
---|---|
solubilized with 3 M urea | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-L-methionyl peptide + H2O | - |
Mycobacterium tuberculosis | formate + methionyl peptide | - |
? | |
N-formyl-L-Met-L-Ala + H2O | - |
Mycobacterium tuberculosis | formate + L-Met-L-Ala | - |
? | |
N-formylmethionine-alanine + H2O | - |
Mycobacterium tuberculosis | formate + methionyl-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mPDF | - |
Mycobacterium tuberculosis |
peptide deformylase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
mPDF, activity assay | Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000197 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | 19.7 nM calculated value for the Mycobacterium tuberculosis enzyme | Mycobacterium tuberculosis |