Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
Fe2+-, Ni2+- and Zn2+-bound forms, hanging and sitting drop vapour diffusion techniques | Escherichia coli |
General Stability | Organism |
---|---|
Ni2+-bound enzyme fully stable, additional stabilization during purification by catalase | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | convertion to the apoenzyme during 1 h at 10 mM | Escherichia coli | |
Zn2+ | tightly bound, very poor activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
N-formyl-Met-Ala | - |
Escherichia coli | |
19 | - |
N-formyl-Met-Ala | Fe2+-bound enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | natural metal, bound to active site | Escherichia coli | |
Fe2+ | sensitive to reactive oxygen | Escherichia coli | |
Ni2+ | gives insensitivity to oxidation | Escherichia coli | |
Zn2+ | Zn2+-bound enzyme inactive in crystallization analysis | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
sensitive to intracellular reactive oxygen | Escherichia coli |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant Ni2+, Fe2+- and Zn2+-bound preparations | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1200 | - |
Fe2+- and Ni2+-bound enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 2 mg Ni2+-bound enzyme/ml, pH 7.7, several weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-Met-Ala + H2O | - |
Escherichia coli | formate + Met-Ala | - |
? | |
N-formyl-Met-Ala-Ser + H2O | - |
Escherichia coli | formate + Met-Ala-Ser | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 19000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2100 | - |
N-formyl-Met-Ala | recombinant Fe2+-bound enzyme | Escherichia coli |