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Literature summary for 3.5.1.88 extracted from
- Identification of regions involved in enzymatic stability of peptide deformylase of Mycobacterium tuberculosis (2005), J. Bacteriol., 187, 8216-8220.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pET28c vector for over-expression in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA182-197 | deletion mutant, amino acids 182-197 were removed from the carboxy-terminal end, inactive | Mycobacterium tuberculosis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mycobacterium tuberculosis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Mycobacterium tuberculosis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22600 | - |
calculated molecular mass | Mycobacterium tuberculosis |
| 31000 | - |
SDS-PAGE, Western blotting using an anti-his tag antibody | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formyl-L-methionyl peptide + H2O | Mycobacterium tuberculosis | - |
formate + methionyl peptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on Ni-NTA column | Mycobacterium tuberculosis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilized with 3 M urea | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formyl-L-methionyl peptide + H2O | - |
Mycobacterium tuberculosis | formate + methionyl peptide | - |
? | |
| N-formylmethionine-alanine + H2O | - |
Mycobacterium tuberculosis | formate + methionyl-alanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | mPDF in solution shows a molecular mass of 100 kDa, several mixed fractions of different mutants display enzyme activity | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mPDF | - |
Mycobacterium tuberculosis |
| peptide deformylase | - |
Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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