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Literature summary for 3.5.1.88 extracted from

  • Nguyen, K.T.; Wu, J.C.; Boylan, J.A.; Gherardini, F.C.; Pei, D.
    Zinc is the metal cofactor of Borrelia burgdorferi peptide deformylase (2007), Arch. Biochem. Biophys., 468, 217-225.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with a C-terminal six-histidine tag (BbPDF1-HT) Borreliella burgdorferi

Inhibitors

Inhibitors Comment Organism Structure
(2R)-N-[(1S)-1-(dimethylcarbamoyl)-2,2-dimethylpropyl]-2-[[formyl(hydroxy)amino]methyl]hexanamide Zn-BbPDF1-HT Borreliella burgdorferi
N-[[(3S,6R)-3-tert-butyl-2,5-dioxo-1,4-diazacyclopentadecan-6-yl]methyl]-N-hydroxyformamide Zn-BbPDF1-HT Borreliella burgdorferi

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ it contains 1.1 Zn2+ ion/polypeptide Borreliella burgdorferi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
x * 22000 + X * 60000, SDS-PAGE Borreliella burgdorferi
60000
-
x * 22000 + X * 60000, SDS-PAGE Borreliella burgdorferi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formyl-L-methionyl peptide + H2O Borreliella burgdorferi peptide deformylase catalyzes the removal of N-terminal formyl groups from nascent ribosome-synthesized polypeptides formate + L-methionyl peptide
-
?

Organism

Organism UniProt Comment Textmining
Borreliella burgdorferi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Borreliella burgdorferi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.235
-
-
Borreliella burgdorferi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-L-Met-Leu-p-nitroanilide + H2O
-
Borreliella burgdorferi formate + Met-Leu-p-nitroanilide
-
?
formyl-L-methionyl peptide + H2O peptide deformylase catalyzes the removal of N-terminal formyl groups from nascent ribosome-synthesized polypeptides Borreliella burgdorferi formate + L-methionyl peptide
-
?

Subunits

Subunits Comment Organism
? x * 22000 + X * 60000, SDS-PAGE Borreliella burgdorferi

Synonyms

Synonyms Comment Organism
BbPDF
-
Borreliella burgdorferi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
N-[[(3S,6R)-3-tert-butyl-2,5-dioxo-1,4-diazacyclopentadecan-6-yl]methyl]-N-hydroxyformamide Zn-BbPDF1-HT Borreliella burgdorferi
0.000039
-
(2R)-N-[(1S)-1-(dimethylcarbamoyl)-2,2-dimethylpropyl]-2-[[formyl(hydroxy)amino]methyl]hexanamide Zn-BbPDF1-HT Borreliella burgdorferi