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3.4.22.60: caspase-7

This is an abbreviated version!
For detailed information about caspase-7, go to the full flat file.

Word Map on EC 3.4.22.60

Reaction

strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-/- =

Synonyms

apoptotic protease Mch-3, C14.004, Casp-7, Casp7, caspase 7, caspase-7, CMH-1, cystein aspartic-specific protease-7, DEVDase, ICE-LAP3, ICE-like apoptotic protease 3, LICE2 cysteine protease, More, SCA-2, SREBP cleavage activity 2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.60 caspase-7

Cloned

Cloned on EC 3.4.22.60 - caspase-7

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bacterially expressed Mch3 has intrinsic autocatalytic and autoactivation activity
cloned into the NcoI site of the pET11d vector and expression in Escherichia coli BL21codon+
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expressed in COS7 cells
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expressed in Escherichia coli BL21(DE3)pLysS cells
expression in CG1945 yeast strain
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expression in Escherichia coli
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expression in Escherichia coli BL21 (DE3) transformed with a pET-21b plasmid expression vector
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expression of C-terminally His-tagged caspase-7 in Escherichia coli strain BL21 (DE3)
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expression of caspase-7 in caspase-7-deficient mouse macrophages
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expression of FLAG-tagged caspase-7 in DKD cells. Simultaneous expression of HA-procaspase-3, EC 3.4.22.56, and HA-procaspase-7 in DKD HeLa cells together with FLAG-caspase-3 or FLAG-caspase-7. HA-procaspase-3 is coimmunoprecipitated with FLAG-procaspase-3 but not with FLAG-procaspase-7. Similarly, HA-procaspase-7 is co-immunoprecipitated with FLAG-procaspase-7 but not with FLAG-procaspase-3
expression of procaspase-7 in Escherichia coli strain BL21(DE3)
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gene CASP-7, expression in Escherichia coli strain BL21(DE3)
His-tagged version expressed in Escherichia coli
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His-tagged version expressed in Escherichia coli BL21
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overexpression in COS cells
quantitative expression analysis in several ovarian and endometrial cancer cell lines by RT-PCR
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RT-PCR enzyme expression analysis in the central nervous system of neuropathic rats
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two truncated mutant isozymes 24casp7 and 57casp7 are cloned from a skeletal muscle cDNA library, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
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