Information on EC 3.4.22.60 - caspase-7

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The expected taxonomic range for this enzyme is: Tetrapoda

EC NUMBER
COMMENTARY hide
3.4.22.60
-
RECOMMENDED NAME
GeneOntology No.
caspase-7
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-/-
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
189258-14-8
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
-
active caspase-7 colocalizes with internalized TNF-R1 receptosomes. TNF-induced caspase-7 activation takes place exclusively within TNF receptosomes
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
26S protease regulatory subunit 4 + H2O
?
show the reaction diagram
-
SwissProt: P62192 M
-
-
?
26S protease regulatory subunit 7 + H2O
?
show the reaction diagram
-
SwissProt: P35998
-
-
?
26S proteasome subunit + H2O
?
show the reaction diagram
-
during apoptosis, the activated caspase-7 directly cleaves certain subunits of 26S proteasomes, which then result in a decline in the proteasomal activity
-
-
?
Ac-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O
Ac-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin
show the reaction diagram
-
a caspase-3 substrate
-
-
?
Ac-DEVD-4-nitroanilide + H2O
Ac-DEVD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Ac-DEVD-7-amido-4-methylcoumarin + H2O
Ac-DEVD + 7-amino-4-methylcoumarin
show the reaction diagram
Ac-DEVD-p-nitroanilide + H2O
p-nitroaniline + Ac-DEVD
show the reaction diagram
-
-
-
-
?
Ac-DQTD-7-amido-4-methylcoumarin + H2O
Ac-DQTD + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Ac-DVAD-p-nitroanilide + H2O
p-nitroaniline + Ac-DVAD
show the reaction diagram
-
-
-
-
?
Ac-LDVAD-p-nitroanilide + H2O
p-nitroaniline + Ac-LDVAD
show the reaction diagram
-
-
-
-
?
Ac-VDVAD-p-nitroanilide + H2O
p-nitroaniline + Ac-VDVAD
show the reaction diagram
-
-
-
-
?
acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin
acetyl-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
acetyl-Asp-Glu-Val-Asp-p-nitroanilide + H2O
acetyl-Asp-Glu-Val-Asp + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-ASTD-7-amido-4-methylcoumarin + H2O
acetyl-ASTD + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
acetyl-DEVD-4-nitroanilide + H2O
acetyl-DEVD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O
acetyl-DEVD + 7-amino-4-fluoromethylcoumarin
show the reaction diagram
-
-
-
-
?
acetyl-DEVD-7-amido-4-methylcoumarin + H2O
acetyl-DEVD + 7-amino-4-methylcoumarin
show the reaction diagram
acetyl-DQMD-4-nitroanilide + H2O
acetyl-DQMD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-VDQQD-4-nitroanilide + H2O
acetyl-VDQQD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-VDQVDGW-amide + H2O
?
show the reaction diagram
-
preferred peptide substrate
-
-
?
acetyl-VDVAD-4-nitroanilide + H2O
acetyl-VAVAD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-VEID-4-nitroanilide + H2O
acetyl-VEID + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-VQVD-4-nitroanilide + H2O
acetyl-VQVD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-VQVDGW-amide + H2O
?
show the reaction diagram
-
preferred peptide substrate
-
-
?
acetyl-YEVD-4-nitroanilide + H2O
acetyl-YEVD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acid sphingomyelinase + H2O
?
show the reaction diagram
actin-related protein 2/3 complex subunit 3 + H2O
?
show the reaction diagram
-
-
-
-
?
ataxin-7 + H2O
?
show the reaction diagram
caspase-7 biosensor vDEVDc + H2O
?
show the reaction diagram
claspin + H2O
?
show the reaction diagram
DEVD-4-nitroanilide + H2O
DEVD + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
DEVD-7-amido-4-methylcoumarin + H2O
DEVD + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
DEVD-7-amido-4-trifluoromethylcoumarin + H2O
DEVD + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
epidermal growth factor receptor + H2O
?
show the reaction diagram
far upstream element binding protein 1 + H2O
?
show the reaction diagram
-
-
-
-
?
FK506-binding protein 4 + H2O
?
show the reaction diagram
-
-
-
-
?
fructose-bisphosphate aldolase A + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-actin + H2O
?
show the reaction diagram
-
-
-
-
?
human telomerase + H2O
?
show the reaction diagram
-
caspase-7 cleaves human telomerase, hTERT, at residues E286 and D628, generation of three major proteolytic fragments, cleavage site determination using caspase-7 resistant substrate mutants D129A, D637A, and D129A/D637A, overview
-
-
?
inhibitor of caspase-actived DNase + H2O
?
show the reaction diagram
Kaposi sarcoma-associated herpesvirus ORF57 + H2O
?
show the reaction diagram
kinectin + H2O
?
show the reaction diagram
lamin B2 + H2O
?
show the reaction diagram
-
-
-
-
?
mammalian sterile 20-like kinase 1 + H2O
?
show the reaction diagram
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O
N-acetyl-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide + H2O
N-acetyl-Asp-Glu-Val-Asp + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O
N-acetyl-DEVD + 7-amino-4-fluoromethylcoumarin
show the reaction diagram
-
-
-
-
?
N-acetyl-DEVD-7-amido-4-methylcoumarin + H2O
N-acetyl-DEVD + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-carbobenzyloxy-DEVD-R110 + H2O
?
show the reaction diagram
-
a fluorescent substrate
-
-
?
Nogo-B + H2O
?
show the reaction diagram
-
-
-
-
?
nucleoside diphosphate kinase B + H2O
?
show the reaction diagram
-
-
-
-
?
p23 + H2O
?
show the reaction diagram
-
better substrate for caspase-7 than caspase-3. p23 is cleaved at site PEVD*G
-
-
?
PARP + H2O
?
show the reaction diagram
pLyn-yellow-fluorescent protein-DEVD-DEVD-C fluorescent protein + H2O
pLyn-yellow-fluorescent protein-DEVD + DEVD-cyan fluorescent protein
show the reaction diagram
-
-
-
-
?
poly(ADP ribose) polymerase 1 + H2O
?
show the reaction diagram
-
better substrate for caspase-7 than caspase-3, PARP-1 is cleaved at the canonical caspase-3/7 site DEVD*G
-
-
?
poly(ADP-ribose) polymerase + H2O
?
show the reaction diagram
poly(ADP-ribose)polymerase + H2O
?
show the reaction diagram
-
-
-
-
?
poly-ADPribose polymerase 1 + H2O
poly-ADPribose polymerase 1 peptide fragments
show the reaction diagram
pro-endothelial monocyte-activating polypeptide II + H2O
?
show the reaction diagram
-
caspase-7-mediated generation and release of mature endothelial monocyte-activating polypeptide II may provide a mechanism for leukocyte recruitment to sites of programmed cell death, and thus may link apoptosis to inflammation
-
-
?
pro-endothelial monocyte-activating polypeptide II + H2O
endothelial monocyte-activating polypeptide II + ?
show the reaction diagram
-
pro-endothelial monocyte-activating polypeptide II in which the ASTD cleavage site is changed to the sequence ASTA, is not processed by caspase-7
-
-
?
proteasome activator complex subunit 1 + H2O
?
show the reaction diagram
-
SwissProt: Q06323
-
-
?
proteasome subunit alpha type-1 + H2O
?
show the reaction diagram
-
SwissProt: P25786
-
-
?
proteasome subunit alpha type-2 + H2O
?
show the reaction diagram
proteasome subunit alpha type-3 + H2O
?
show the reaction diagram
-
SwissProt: P25788
-
-
?
proteasome subunit alpha type-6 + H2O
?
show the reaction diagram
-
SwissProt: P60900
-
-
?
septin 5 + H2O
?
show the reaction diagram
-
i.e. peanut-like protein 1
-
-
?
serine/threonine-protein kinase PAK2 + H2O
?
show the reaction diagram
-
-
-
-
?
SETbeta + H2O
?
show the reaction diagram
-
-
-
-
?
sterol regulatory element binding protein-2 + H2O
?
show the reaction diagram
SREBP-2 is sterol regulatory element binding protein-2
-
-
?
TAR DNA binding protein 43 + H2O
?
show the reaction diagram
-
-
-
-
?
thioredoxin peroxidase 1 + H2O
?
show the reaction diagram
-
-
-
-
?
tumor necrosis factor receptor-I + H2O
?
show the reaction diagram
-
mutation E260Q of tumor necrosis factor receptor-I is sufficient to prevent cleavage
-
-
?
valosin-containing protein + H2O
?
show the reaction diagram
VDVAD-7-amido-4-methylcoumarin + H2O
VDVAD + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
vimentin + H2O
?
show the reaction diagram
-
-
-
-
?
viral nucleocapsid protein of transmissible gastroenteritis coronovirus + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
26S proteasome subunit + H2O
?
show the reaction diagram
-
during apoptosis, the activated caspase-7 directly cleaves certain subunits of 26S proteasomes, which then result in a decline in the proteasomal activity
-
-
?
acid sphingomyelinase + H2O
?
show the reaction diagram
-
caspase-7 activates acid sphingomyelinase induced by TNF, overview. Generation of the active 57 kDa fragment of endogenous A-SMase in wild-type HeLa cells. Direct association of caspase-7 and A-SMase within TNF receptosomes
-
-
?
actin-related protein 2/3 complex subunit 3 + H2O
?
show the reaction diagram
-
-
-
-
?
ataxin-7 + H2O
?
show the reaction diagram
-
ataxin-7 is cleaved by caspase-7, cleavage sites to Asp residues at positions 266 and 344 of the ataxin-7 protein. Proteolytic processing of ataxin-7 by caspase-7 may contribute to SCA7 disease pathogenesis
-
-
?
caspase-7 biosensor vDEVDc + H2O
?
show the reaction diagram
-
wild-type caspase-7 binds to caspase-7 biosensor vDEVDc, but does not cleave it, while an mutant isozymes of caspase-7 with the 24 or 56 N-terminal residues truncated, i.e. 24casp7 and 57casp7, respectively, cleave vDEVDc at the recognition sequence
-
-
?
epidermal growth factor receptor + H2O
?
show the reaction diagram
-
cleavage during apoptosis
-
-
?
far upstream element binding protein 1 + H2O
?
show the reaction diagram
-
-
-
-
?
FK506-binding protein 4 + H2O
?
show the reaction diagram
-
-
-
-
?
fructose-bisphosphate aldolase A + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-actin + H2O
?
show the reaction diagram
-
-
-
-
?
Kaposi sarcoma-associated herpesvirus ORF57 + H2O
?
show the reaction diagram
-
a viral early protein essential for KSHV multiplication. Cleavage of wild-type or mutant D33A ORF57 by valproate-induced BCBL-1 cell extracts containing active caspases
-
-
?
kinectin + H2O
?
show the reaction diagram
-
kinectin is cleaved by caspase 7 during apoptosis induced by different stimuli. Kinectin functions as a membrane anchor for kinesin and may be relevant to the disruption of vesicle trafficking during apoptosis
-
-
?
lamin B2 + H2O
?
show the reaction diagram
-
-
-
-
?
mammalian sterile 20-like kinase 1 + H2O
?
show the reaction diagram
-
i.e. Mst1. Caspase 7 preferentially activates c-Jun NH2-terminal kinase and p38 preferentially through 40000 Da cleaved forms of Mst1. The 40000 Da form of Mst1 selectively phosphorylates c-Jun NH2-terminal kinase and p38
-
-
?
nucleoside diphosphate kinase B + H2O
?
show the reaction diagram
-
-
-
-
?
p23 + H2O
?
show the reaction diagram
-
better substrate for caspase-7 than caspase-3. p23 is cleaved at site PEVD*G
-
-
?
PARP + H2O
?
show the reaction diagram
-
-
-
-
?
poly(ADP ribose) polymerase 1 + H2O
?
show the reaction diagram
-
better substrate for caspase-7 than caspase-3, PARP-1 is cleaved at the canonical caspase-3/7 site DEVD*G
-
-
?
poly(ADP-ribose) polymerase + H2O
?
show the reaction diagram
poly-ADPribose polymerase 1 + H2O
poly-ADPribose polymerase 1 peptide fragments
show the reaction diagram
-
-
i.e. c-PARP, frgaments of 24 and 89 kDa
-
?
pro-endothelial monocyte-activating polypeptide II + H2O
?
show the reaction diagram
-
caspase-7-mediated generation and release of mature endothelial monocyte-activating polypeptide II may provide a mechanism for leukocyte recruitment to sites of programmed cell death, and thus may link apoptosis to inflammation
-
-
?
proteasome subunit alpha type-2 + H2O
?
show the reaction diagram
-
-
-
-
?
septin 5 + H2O
?
show the reaction diagram
-
i.e. peanut-like protein 1
-
-
?
serine/threonine-protein kinase PAK2 + H2O
?
show the reaction diagram
-
-
-
-
?
SETbeta + H2O
?
show the reaction diagram
-
-
-
-
?
TAR DNA binding protein 43 + H2O
?
show the reaction diagram
-
-
-
-
?
thioredoxin peroxidase 1 + H2O
?
show the reaction diagram
-
-
-
-
?
valosin-containing protein + H2O
?
show the reaction diagram
-
cleavage by caspase-7 during apoptosis
-
-
?
vimentin + H2O
?
show the reaction diagram
-
-
-
-
?
viral nucleocapsid protein of transmissible gastroenteritis coronovirus + H2O
?
show the reaction diagram
-
cleavage site VVPD359-/-. Destruction of viral protein by the host cell death machinery
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(S)-1-(11,11-difluoroundecyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(2-fluoroallyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(2-fluoroethyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(3-chloro-2-hydroxypropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(3-chloropropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(3-fluorobutyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(3-fluoropropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(3-hydroxypropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(4-fluorobutyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-(4-hydroxybutyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-butyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-ethyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-[3-(3-hydroxypropoxy)propyl]-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-1-[3-[3-(2-fluoroethoxy)propoxy]propyl]-5-[1-(2-methoxymethylpyrrolidinyl)-sulfonyl]isatin
-
-
(S)-3,3-difluoro-1-(3-fluoropropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]indolin-2-one
-
-
(S)-3,5-bis-trifluoromethylbenzyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-3-[5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-2,3-dioxoindolin-1-yl]propanoic acid
-
-
(S)-3-[5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-2,3-dioxoindolin-1-yl]propyl methanesulfonate
-
-
(S)-4-fluorobenzyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-4-trifluoromethylbenzyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
(S)-4-[5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-2,3-dioxoindolin-1-yl]butyl 4-methyl-benzenesulfonate
-
-
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-(3,3,3-trifluoropropyl)isatin
-
-
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-(3,4,4-trifluorobut-3-enyl)isatin
-
-
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-(4,4,4-trifluorobutyl)isatin
-
-
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-methylisatin
-
-
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-propylisatin
-
-
5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
-
-
AC-DEVD-aldehyde inhibitor
-
-
Ac-DEVD-CHO
Ac-DEVD-fluoromethylketone
-
a caspase-7 inhibitor
AC-DQTD-CHO
-
-
Ac-Val-Ala-Asp-fluoromethylketone
-
-
acetyl-AEVD-aldehyde
-
-
acetyl-Ala-Pro-Nle-Asp-aldehyde
-
-
acetyl-DEVD-aldehyde
-
-
acetyl-IETD-aldehyde
-
-
Bcl2L12
-
is a direct caspase-7-binding and inhibitory protein affecting effector caspase activation, knockdown of Bcl2L12 or to a lesser extent of XIAP and Bcl-2 results in enhanced caspase-7 activation, regulation, overview
-
benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethyl ketone
-
-
benzyloxycarbonyl-ASTD-fluoromethylketone
-
0.01 mM, complete inhibition of cleavage of pro-endothelial monocyte-activating polypeptide II
benzyloxycarbonyl-DEVD-chloromethylketone
-
0.01 mM, complete inhibition
benzyloxycarbonyl-Pro-Nle-Asp-aldehyde
-
-
-
benzyloxycarbonyl-VAD-fluoromethylketone
-
t1/2 at 0.001 mM is 98 s
benzyloxycarbonyl-VAD-[(2,6-dichlorobenzoyl)-oxy]methyl ketone
-
-
-
benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone
-
-
Boc-Asp-fluoromethylketone
-
-
-
carbobenzyloxy-DEVD-fluoromethyl ketone
-
blocks activation of caspase-7
cowpox seroin CrmA
-
very weak inhibitor
-
DEVD-aldehyde
-
potent inhibitor
DEVD-CHO
-
-
DEVD-fluoromethylketone
-
more specific than YVAD-cmk
ketonic peptides
-
in the straight-chain aliphatic series, increasing inhibition with increasing chain length, for the unsubstituted aromatic P1' inhibitors increasing potency with decreasing linker length
-
N-acetyl-Trp-Glu-His-Asp-CHO
-
-
N-acetyl-Tyr-Val-Ala-Asp-CHO
-
-
N-alpha-tosyl-L-lysyl-chloromethylketone
-
i.e. TLCK, substantially inhibits caspase-7 enzymatic DEVDase activity, but does not affect caspase-7 processing in cells exposed to pro-apoptotic inducing stimuli, irreversibly alkylates cysteine residues of the active site of cysteine proteases
N-carbobenzyloxy-VAD-fluoromethyl ketone
-
a general caspase inhibitor
N-tosyl-L-phenylalanyl-chloromethylketone
-
i.e. TPCK, enhances caspase-7 processing although it substantially inhibits caspase-7 enzymatic DEVDase activity in HL-60 cells exposed to various cell death inducing stimuli, irreversibly alkylates cysteine residues of the active site of cysteine proteases
X-linked inhibitor of apoptosis
-
-
-
XIAP inhibitors
-
peptide inhibitors
-
YVAD-aldehyde
-
weak inhibitor
YVAD-chloromethylketone
-
-
Z-DEVD-fluoromethylketone
Z-VAD-fluoromethylketone
Z-Val-Ala-Asp-fluoromethylketone
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(4-methoxyphenyl)-4-(piperazin-1-yl)quinoline
-
9% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
2-(4-methoxyphenyl)-N-[2-(morpholin-4-yl)ethyl]quinolin-4-amine
-
4% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
apoptosome complex
-
-
-
caspase 1
-
caspase-7 is cleaved and activated by caspase-1. This is mediated by flagellin and requires the host receptors Nlrc4 and Naip5
-
ceramide
-
-
cytolethal distending toxin
-
from Actinobacillus actinomycetemcomitans
-
etoposide
FSH
-
antiapoptotic effect on granulosa cells and a proapoptotic effect on theca-interstitial cells
gonadotropins
-
increases caspase-7 activity in both theca-interstitial cells and granulosa cells
-
LH
-
antiapoptotic effect on granulosa cells and a proapoptotic effect on theca-interstitial cells
-
N'-[2-(4-ethylphenyl)quinolin-4-yl]-N,N-dimethylethane-1,2-diamine
-
17% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
N'-[2-(4-ethylphenyl)quinolin-4-yl]-N,N-dimethylpropane-1,3-diamine
-
26% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
N'-[2-(4-methoxyphenyl)quinolin-4-yl]-N,N-dimethylethane-1,2-diamine
-
123% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
N'-[2-(4-methoxyphenyl)quinolin-4-yl]-N,N-dimethylpropane-1,3-diamine
-
117% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
N,N-diethyl-N'-[2-(4-ethylphenyl)quinolin-4-yl]propane-1,3-diamine
-
24% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
N,N-diethyl-N'-[2-(4-methoxyphenyl)quinolin-4-yl]propane-1,3-diamine
-
124% stimulation of caspase-7 cleavage of TDP-43 at 0.01 mM
N6-(2-isopentenyl)adenosine
-
highly induces enzyme activation
nitric oxide
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2193
Ac-DVAD-p-nitroanilide
-
-
0.3239
Ac-LDVAD-p-nitroanilide
-
-
0.3149
Ac-VDVAD-p-nitroanilide
-
-
0.012 - 0.0646
acetyl-DEVD-4-nitroanilide
0.0605
acetyl-DEVD-7-amido-4-fluoromethylcoumarin
-
pH 7.2, 37C
0.015 - 0.1
acetyl-DEVD-7-amido-4-methylcoumarin
0.13
acetyl-DQMD-4-nitroanilide
-
pH 7.5, 30C
3.1
acetyl-VDQQD-4-nitroanilide
-
pH 7.5, 30C
0.125
acetyl-VDQVDGW-amide
-
pH 7.5, 30C
0.2
acetyl-VDVAD-4-nitroanilide
-
pH 7.5, 30C
0.13
acetyl-VQVDGW-amide
-
pH 7.5, 30C
0.57
acetyl-YEID-4-nitroanilide
-
pH 7.5, 30C
0.49
acetyl-YEVD-4-nitroanilide
-
pH 7.5, 30C
2.1
acetyl-YQVD-4-nitroanilide
-
pH 7.5, 30C
0.0848 - 0.9365
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide
0.0298 - 0.0687
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin
additional information
additional information
-
binding between the mutant 24casp7 isozyme and the vDEVDc biosensor occurs exponentially
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9467 - 6.08
Ac-DVAD-p-nitroanilide
1.102 - 6.08
Ac-LDVAD-p-nitroanilide
1.26
Ac-VDVAD-p-nitroanilide
Homo sapiens
-
-
6.9
acetyl-DEVD-7-amido-4-fluoromethylcoumarin
Homo sapiens
-
pH 7.2, 37C
5.5 - 6.3
acetyl-DEVD-7-amido-4-methylcoumarin
10.3
acetyl-DEVD-p-nitroanilide
Homo sapiens
-
pH 7.2, 37C
0.008 - 4.4
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide
2.9 - 9.5
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin
additional information
additional information
Homo sapiens
-
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009 - 51.7
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide
7048
86 - 140
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin
202476
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000425
acetyl-AEVD-aldehyde
-
pH 7.5, 25C
0.133
acetyl-Ala-Pro-Nle-Asp-aldehyde
-
pH 7.5
0.0000016 - 0.000035
acetyl-DEVD-aldehyde
0.00328
acetyl-IETD-aldehyde
-
pH 7.5, 25C
0.126
benzyloxycarbonyl-Pro-Nle-Asp-aldehyde
-
pH 7.5, 25C
-
0.0000018
DEVD-aldehyde
-
pH 7.5, 37C
0.0044
N-acetyl-Trp-Glu-His-Asp-CHO
-
-
0.0124
N-acetyl-Tyr-Val-Ala-Asp-CHO
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000111
(S)-1-(11,11-difluoroundecyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000266
(S)-1-(2-fluoroallyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.00294
(S)-1-(2-fluoroethyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000123
(S)-1-(3-chloro-2-hydroxypropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000079
(S)-1-(3-chloropropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.0000068
(S)-1-(3-fluorobutyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.00144
(S)-1-(3-fluoropropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000052
(S)-1-(3-hydroxypropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000028
(S)-1-(4-fluorobutyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000011
(S)-1-(4-hydroxybutyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.0000081
(S)-1-butyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000319
(S)-1-ethyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.113
(S)-1-[3-(3-hydroxypropoxy)propyl]-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.0194
(S)-1-[3-[3-(2-fluoroethoxy)propoxy]propyl]-5-[1-(2-methoxymethylpyrrolidinyl)-sulfonyl]isatin
Homo sapiens
-
-
0.00564
(S)-3,3-difluoro-1-(3-fluoropropyl)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]indolin-2-one
Homo sapiens
-
-
0.000135
(S)-3,5-bis-trifluoromethylbenzyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000129
(S)-3-[5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-2,3-dioxoindolin-1-yl]propanoic acid
Homo sapiens
-
-
0.000217
(S)-3-[5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-2,3-dioxoindolin-1-yl]propyl methanesulfonate
Homo sapiens
-
-
0.000044
(S)-4-fluorobenzyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000011
(S)-4-trifluoromethylbenzyl-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
0.000029
(S)-4-[5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-2,3-dioxoindolin-1-yl]butyl 4-methyl-benzenesulfonate
Homo sapiens
-
-
0.00004
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-(3,3,3-trifluoropropyl)isatin
Homo sapiens
-
-
0.000178
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-(3,4,4-trifluorobut-3-enyl)isatin
Homo sapiens
-
-
0.0001
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-(4,4,4-trifluorobutyl)isatin
Homo sapiens
-
-
0.000304
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-methylisatin
Homo sapiens
-
-
0.000058
(S)-5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]-1-propylisatin
Homo sapiens
-
-
0.00017
5-[1-(2-methoxymethylpyrrolidinyl)sulfonyl]isatin
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
apoptotic activity of active caspase-7, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
cleavage of sterol regulatory element binding protein
7.2
-
assay at
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
pH 6.0: about 35% of maximal activity, pH 7.0: about 15% of maximal activity, cleavage of sterol regulatory element binding protein
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
endometrial carcinoma cell line
Manually annotated by BRENDA team
-
primary
Manually annotated by BRENDA team
-
B cells derived from cavity-based B cell lymphoma
Manually annotated by BRENDA team
-
B cells derived from cavity-based B cell lymphoma
Manually annotated by BRENDA team
-
the activated form of caspase-7 is detected from the beginning of ossification during embryonic development and persists postnatally in alveolar and mandibular bones as well as long bones of limbs
Manually annotated by BRENDA team
-
conjunctival cell
Manually annotated by BRENDA team
-
endometrial carcinoma cell line
Manually annotated by BRENDA team
-
endometrial carcinoma cell line
Manually annotated by BRENDA team
-
ovarian epithelial cell line
Manually annotated by BRENDA team
-
ovarian epithelial cell line
Manually annotated by BRENDA team
-
ovarian epithelial cell line
Manually annotated by BRENDA team
-
monolayer cell culture
Manually annotated by BRENDA team
-
monolayer cell culture
Manually annotated by BRENDA team
-
a glioma cell line
Manually annotated by BRENDA team
-
monolayer cell culture
Manually annotated by BRENDA team
-
ovarian carcinoma cell line
Manually annotated by BRENDA team
-
ovarian carcinoma cell line
Manually annotated by BRENDA team
-
ovarian carcinoma cell line
Manually annotated by BRENDA team
-
ovarian carcinoma cell line
Manually annotated by BRENDA team
-
endometrial carcinoma cell line
Manually annotated by BRENDA team
-
increased expression of caspase-7 mRNA in the dorsal horn spinal cord by 3 days post-chronic constrictive injury
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
ovarian carcinoma cell line
Manually annotated by BRENDA team
-
stage 62 tadpole tail
Manually annotated by BRENDA team
-
stage 62 tadpole tail
Manually annotated by BRENDA team
-
precursor neurons
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
juxtamembrane structures
Manually annotated by BRENDA team
-
spheroids of HT-29 cells
-
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
1 * 31000 + 1 * 20000, active caspase-7, SDS-PAGE
26000
-
active form of caspase-7, Western blot analysis
31000
-
1 * 31000 + 1 * 20000, active caspase-7, SDS-PAGE
32000
-
procaspase-7, Western blot analysis
35000
-
2 * 35000, procaspase-7 C285A mutant, in the homodimeric procaspase-7 each monomer is organized in two structured subdomains connected by partially flexible linkers, which asymmetrically occupy and block the central cavity, SDS-PAGE
60000
-
procaspase-7, immunoblotting
200000
-
X-linked inhibitor of apoptosis-caspase-7 complex, immunoblotting
250000
-
Western blot analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
-
monomer
-
1 * 35000-37000, procaspase-7, SDS-PAGE
tetramer
-
-
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2.9 A crystal structure of recombinant C285A procaspase, sitting drop vapor diffusion method
-
hanging drop method, crystal structures of caspase-7 are determined in complexes with six peptide analogs (acetyl-DMQD-CHO, acetyl-DQMD-CHO, acetyl-DNLD-CHO, acetyl-IEPD-CHO, acetyl-ESMD-CHO, acetyl-WEHD-CHO)
-
hanging drop vapor diffusion method
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by nickel affinity chromatography, anion exchange chromatography and gel filtration
-
commercial preparation
-
immobilized metal affinity column chromatography
-
immobilized metal ion affinity chromatography (Co2+)
-
immobilized metal ion affinity chromatography (Ni2+), gel filtration
-
partially purified X-linked inhibitor of apoptosis-caspase-7 complex by gel filtration and immunopurification, SDS-PAGE
-
recombinant C-terminally His-tagged caspase-7 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinant procaspase-2 from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 95%
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
bacterially expressed Mch3 has intrinsic autocatalytic and autoactivation activity
-
cloned into the NcoI site of the pET11d vector and expression in Escherichia coli BL21codon+
-
expressed in COS7 cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expression in CG1945 yeast strain
-
expression in Escherichia coli
-
expression in Escherichia coli BL21 (DE3) transformed with a pET-21b plasmid expression vector
-
expression of C-terminally His-tagged caspase-7 in Escherichia coli strain BL21 (DE3)
-
expression of caspase-7 in caspase-7-deficient mouse macrophages
-
expression of FLAG-tagged caspase-7 in DKD cells. Simultaneous expression of HA-procaspase-3, EC 3.4.22.56, and HA-procaspase-7 in DKD HeLa cells together with FLAG-caspase-3 or FLAG-caspase-7. HA-procaspase-3 is coimmunoprecipitated with FLAG-procaspase-3 but not with FLAG-procaspase-7. Similarly, HA-procaspase-7 is co-immunoprecipitated with FLAG-procaspase-7 but not with FLAG-procaspase-3
-
expression of procaspase-7 in Escherichia coli strain BL21(DE3)
-
gene CASP-7, expression in Escherichia coli strain BL21(DE3)
His-tagged version expressed in Escherichia coli
-
His-tagged version expressed in Escherichia coli BL21
-
overexpression in COS cells
-
quantitative expression analysis in several ovarian and endometrial cancer cell lines by RT-PCR
-
RT-PCR enzyme expression analysis in the central nervous system of neuropathic rats
-
two truncated mutant isozymes 24casp7 and 57casp7 are cloned from a skeletal muscle cDNA library, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C186S
-
site-directed mutagenesis
C285A
-
mutant procaspase-7 shows no autoactivation
D198A
-
additionally, amino acid residues 20-25 are replaced with a thrombin cleavage site (LVPRGS) and a thrombin cleavage site (LVPRGS) is inserted between Asp-203 and Thr-204
D23A
-
the mutation shows increased catalytic activity but is unable to cleave PARP
D628A
-
site-directed mutagenesis, caspase-7 resistant mutant, retains telomerase activity
E286A
-
site-directed mutagenesis, inactive mutant
E286A/D628A
-
site-directed mutagenesis, inactive mutant
K38A
-
the mutant shows increased catalytic activity
K40A
-
the mutant shows reduced catalytic activity
K41A
-
the mutant shows reduced catalytic activity
S37A
-
the mutant shows increased catalytic activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
molecular biology
-
engineering of CHO cells for more robust cell lines includes reduction of apoptotic capase-7, overview
additional information
-
apoptosis is preceded by proteolytic cleavage of e.g. caspase 7, prolonged nuclear localization of activated signal transducer and activator of transcription 1 results in apoptosis involving specific regulation of caspase pathway