Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.60 extracted from

  • Majerciak, V.; Kruhlak, M.; Dagur, P.K.; McCoy, J.P.; Zheng, Z.M.
    Caspase-7 cleavage of Kaposi sarcoma-associated herpesvirus ORF57 confers a cellular function against viral lytic gene expression (2010), J. Biol. Chem., 285, 11297-11307.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.60

Protein Variants

Protein Variants Comment Organism
additional information enzyme knockdown by caspase-7 siRNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Z-DEVD-fluoromethylketone a caspase-3 and caspase-7 inhibitor Homo sapiens
Z-VAD-fluoromethylketone a pan-caspase inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Kaposi sarcoma-associated herpesvirus ORF57 + H2O Homo sapiens a viral early protein essential for KSHV multiplication. Cleavage of wild-type or mutant D33A ORF57 by valproate-induced BCBL-1 cell extracts containing active caspases ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
B-lymphocyte B cells derived from cavity-based B cell lymphoma Homo sapiens
-
B-lymphoma cell B cells derived from cavity-based B cell lymphoma Homo sapiens
-
BCBL-1 cell
-
 Homo sapiens
-
HEK-293 cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Kaposi sarcoma-associated herpesvirus ORF57 + H2O a viral early protein essential for KSHV multiplication. Cleavage of wild-type or mutant D33A ORF57 by valproate-induced BCBL-1 cell extracts containing active caspases Homo sapiens ?
-
 ?
Kaposi sarcoma-associated herpesvirus ORF57 + H2O a viral early protein essential for KSHV multiplication, caspase-7 cleavage site is 30DETD33 Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction depletion of caspase-7 from the cytoplasmic extracts of valproate-induced BCBL-1 cells reduces the cleavage of ORF57, and inhibition of caspase-7 activity promotes the expression of ORF59 Homo sapiens
physiological function caspase-7 cleavage of Kaposi sarcoma-associated herpesvirus ORF57 in the cytoplasm confers a cellular function against viral lytic gene expression. But only initiator caspase-8, EC 3.4.22.61, is triggered in BCBL-1 cells with lytic KSHV induction by valproate, along with cleavage of expressed ORF57. Caspase-7 is an essential caspase in the cleavage of KSHV ORF57 and plays a key role in ORF57 cleavage in BCBL-1 cells during KSHV lytic infection Homo sapiens