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3.4.22.60: caspase-7

This is an abbreviated version!
For detailed information about caspase-7, go to the full flat file.

Word Map on EC 3.4.22.60

Reaction

strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-/- =

Synonyms

apoptotic protease Mch-3, C14.004, Casp-7, Casp7, caspase 7, caspase-7, CMH-1, cystein aspartic-specific protease-7, DEVDase, ICE-LAP3, ICE-like apoptotic protease 3, LICE2 cysteine protease, More, SCA-2, SREBP cleavage activity 2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.60 caspase-7

Purification

Purification on EC 3.4.22.60 - caspase-7

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by nickel affinity chromatography, anion exchange chromatography and gel filtration
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commercial preparation
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immobilized metal affinity column chromatography
immobilized metal ion affinity chromatography (Co2+)
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immobilized metal ion affinity chromatography (Ni2+), gel filtration
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partially purified X-linked inhibitor of apoptosis-caspase-7 complex by gel filtration and immunopurification, SDS-PAGE
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recombinant C-terminally His-tagged caspase-7 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
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recombinant procaspase-2 from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 95%
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