Information on EC 3.4.24.3 - microbial collagenase

Six species of metalloendopeptidase acting on native collagen can be isolated from the medium of Clostridium histolyticum. Class I has forms α (68 kDa), β (115 kDa) and γ (79 kDa); class II has δ (100 kDa), ε (110 kDa) and ζ (125 kDa). The two classes are immunologically crossreactive, but have significantly different sequences, and different specificities such that their actions on collagen are complementary. The enzymes also act as peptidyl-tripeptidases. Variants of the enzyme have been purified from Bacillus cereus , Empedobacter collagenolyticum , Pseudomonas marinoglutinosa , and species of Vibrio, Vibrio B-30 (ATCC 21250) and V. alginolyticus (previously Achromobacter iophagus) [3,8]. Also known from Streptomyces sp. . The Vibrio enzyme is the type example of peptidase family M9.