Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.3 extracted from

  • Sekiguchi, H.; Uchida, K.; Inoue, G.; Matsushita, O.; Saito, W.; Aikawa, J.; Tanaka, K.; Fujimaki, H.; Miyagi, M.; Takaso, M.
    Acceleration of bone formation during fracture healing by poly(pro-hyp-gly)10 and basic fibroblast growth factor containing polycystic kidney disease and collagen-binding domains from Clostridium histolyticum collagenase (2016), J. Biomed. Mater. Res. A, 104, 1372-1378 .
    View publication on PubMed
Search for more literature for 3.4.24.3

Protein Variants

Protein Variants Comment Organism
additional information development of a collagen-like polypeptide-based bone formation system consisting of poly(Pro-Hyp-Gly)10, which mimics the triple helical conformation of collagen, and basic fibroblast growth factor (bFGF) fused to the polycystic kidney disease (PKD) domain and collagen-binding domain (CBD) of Clostridium histolyticum collagenase. The synthetic construct structure is more thermostable (retains structure at up to 80°C) than the native pepsin-soluble bovine type I collagen (loses structure at 50°C). The combination of the collagen binding bFGF fusion protein (bFGF-PKD-CBD) with poly(Pro-Hyp-Gly)10 induces greater bone formation compared to bFGF alone in mice bone fracture models. Method evaluation, overview Hathewaya histolytica

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica Q46085
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Subunits

Subunits Comment Organism
More Clostridium histolyticum class II collagenase (ColH) contains two polycystic kidney disease (PKD) domains and one collagenbinding domain (CBD), which are necessary for the catalytic activity and collagen-binding affinity of this enzyme Hathewaya histolytica

Synonyms

Synonyms Comment Organism
class II collagenase
-
 Hathewaya histolytica
ColH
-
 Hathewaya histolytica
collagenase
-
 Hathewaya histolytica

General Information

General Information Comment Organism
additional information development of a collagen-like polypeptide-based bone formation system consisting of poly(Pro-Hyp-Gly)10, which mimics the triple helical conformation of collagen, and basic fibroblast growth factor (bFGF) fused to the polycystic kidney disease (PKD) domain and collagen-binding domain (CBD) of Clostridium histolyticum collagenase. The synthetic construct structure is more thermostable (retains structure at up to 80°C) than the native pepsin-soluble bovine type I collagen (loses structure at 50°C). The combination of the collagen binding bFGF fusion protein (bFGF-PKD-CBD) with poly(Pro-Hyp-Gly)10 induces greater bone formation compared to bFGF alone in mice bone fracture models Hathewaya histolytica