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Literature summary for 3.4.24.3 extracted from

  • Chakraborty, R.; Chandra, A.L.
    Purification and characterization of a streptomycete collagenase (1986), J. Appl. Bacteriol., 61, 331-337.
    View publication on PubMed
Search for more literature for 3.4.24.3

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Streptomyces sp.
8-hydroxyquinoline
-
 Streptomyces sp.
alpha,alpha-dipyridyl
-
 Streptomyces sp.
Cd2+
-
 Streptomyces sp.
glutathione reduced Streptomyces sp.
Mn2+
-
 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Streptomyces sp.
Mg2+ activates Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 1 * 75000, no dissociation into subunits after lithium chloride treatment, SDS-PAGE Streptomyces sp.
75000
-
 ? * 75000, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Streptomyces sp. degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg + H2O
-
 Streptomyces sp. 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
 ?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Streptomyces sp. Hydrolyzed collagen
-
 ?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Streptomyces sp. ?
-
 ?

Subunits

Subunits Comment Organism
? ? * 75000, SDS-PAGE Streptomyces sp.
monomer 1 * 75000, no dissociation into subunits after lithium chloride treatment, SDS-PAGE Streptomyces sp.