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Literature summary for 3.4.24.3 extracted from
- Purification and characterization of a marine bacterial collagenase (1978), Biochemistry, 17, 2857-2863.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate buffer | - |
Vibrio sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol | Vibrio sp. |
| 28000 | - |
x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol | Vibrio sp. |
| 105000 | - |
gel filtration | Vibrio sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | Vibrio sp. | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | ? | - |
? |  |
| Collagen + H2O | Vibrio sp. B-30 | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio sp. | - |
B-30 | - |
| Vibrio sp. B-30 | - |
B-30 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Vibrio sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Vibrio sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Vibrio sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 24 h, pH 4.4-7.4 | Vibrio sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form | Vibrio sp. | Hydrolyzed collagen | - |
? | |
| Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form | Vibrio sp. B-30 | Hydrolyzed collagen | - |
? | |
| Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | Vibrio sp. | ? | - |
? | |
| Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | Vibrio sp. B-30 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol | Vibrio sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Vibrio sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Vibrio sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | 7.4 | 4°C, 24 h | Vibrio sp. |
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