Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, structure of the collagen-binding domain with an N-terminal domain linker from class I collagenase, determined at 1.0 A resolution in the absence of Ca2+ and at 1.65 A resolution in the presence of calcium | Hathewaya histolytica |
Protein Variants | Comment | Organism |
---|---|---|
D904A | mutant enzyme with increased MW in presence of Ca2+ and decreased MW in presence of 1 mM EGTA, determined by gel filtration | Hathewaya histolytica |
D927A | mutant enzyme with increased MW in presence of Ca2+ and slightly decreased MW in presence of 1 mM EGTA, determined by gel filtration | Hathewaya histolytica |
D930A | mutant enzyme with increased MW in presence of Ca2+ and slightly decreased MW in presence of 1 mM EGTA, determined by gel filtration | Hathewaya histolytica |
E899A | mutant enzyme with increased MW in presence of Ca2+ and decreased MW in presence of 1 mM EGTA, determined by gel filtration | Hathewaya histolytica |
E901A | mutant enzyme with increased MW in presence of Ca2+ and decreased MW in presence of 1 mM EGTA, determined by gel filtration | Hathewaya histolytica |
N903A | mutant enzyme with increased MW in presence of Ca2+ and slightly increased MW in presence of 1 mM EGTA, determined by gel filtration | Hathewaya histolytica |
Q972A | mutant enzyme with slightly decreased MW in presence of Ca2+ | Hathewaya histolytica |
S906A | mutant enzyme with slightly decreased MW in presence of Ca2+ | Hathewaya histolytica |
S906F | mutant enzyme with slightly decreased MW in presence of Ca2+ ad slightly increased MW in presence of 1 mM EGTA, determined by gel filtration | Hathewaya histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | conformational change of the linker upon calcium binding in the structure of the collagen-binding domain with an N-terminal domain linker from class I collagenase | Hathewaya histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11470 | - |
mutant enzyme S906F in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
11750 | - |
mutant enzyme S906A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
11810 | - |
mutant enzyme Q972A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
11910 | - |
mutant enzyme V973A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
11920 | - |
wild-type enzyme in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
13720 | - |
mutant enzyme E901A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
13750 | - |
mutant enzyme D904A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
14390 | - |
mutant enzyme E899A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
15370 | - |
mutant enzyme D930A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
15420 | - |
mutant enzyme D930A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
15730 | - |
mutant enzyme N903A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
15790 | - |
mutant enzyme D927A in presence of 1 mM Ca2+, gel filtration | Hathewaya histolytica |
15890 | - |
mutant enzyme E899A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
16160 | - |
mutant enzyme D904A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
16190 | - |
mutant enzyme D927A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
16410 | - |
mutant enzyme S906A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
16430 | - |
wild-type enzyme in presence of 1 mM EGTA, gel filtration | Hathewaya histolytica |
16470 | - |
mutant enzyme Q972A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
16510 | - |
mutant enzyme V973A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
16530 | - |
mutant enzyme N903A in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
16650 | - |
mutant enzyme S906F in presence of 1 mM EDTA, gel filtration | Hathewaya histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hathewaya histolytica | - |
class I collagenase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the N-terminal region of the collagen-binding region is not an essential structural component for collagen binding or recognition but rather a cation-controlled multiconformational domain-linker | Hathewaya histolytica | ? | - |
? |