Literature summary for 3.4.24.3 extracted from

Uchida, K.; Matsushita, O.; Nishi, N.; Inoue, G.; Horikawa, K.; Takaso, M.
Enhancement of periosteal bone formation by basic fibroblast-derived growth factor containing polycystic kidney disease and collagen-binding domains from Clostridium histolyticum collagenase (2015), J. Tissue Eng. Regen. Med., 11, 1165-1172 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a fusion protein of class II collagenase (ColH) fused to basic fibroblast growth factor (recombinant human bFGF), a potent mitogen for mesenchymal cells that accelerates bone union and repair when applied locally at defect sites. The fusion protein markedly enhances bone formation when loaded onto collagen materials used for grafting (from porcine-derived insoluble type I collagen sheet). Also construction of collagen-binding domain (CBD) and polycystic kidney disease (PKD) domain of Clostridium histolyticum class II collagenase (ColH) fused to bFGF, the fusion protein demonstrates that the fusion protein markedly enhances bone formation when loaded onto collagen materials used for grafting. A fusion protein consisting of parathyroid hormone (PTH) and a CBD is shown to accelerate bone formation in an osteoporosis model more rapidly than treatment with a PTH-PKD-CBD fusion protein after systemic injection. Comparison of the biological properties of two collagen-binding forms of bFGF, bFGF-CBD and bFGF-PKD-CBD. Both fusion proteins promote the in vitro proliferation of periosteal mesenchymal cells, indicating that they have biological activity similar to that of native bFGF. In vivo periosteal bone formation assays in rat femurs showed that both bFGF-CBD and bFGF-PKD-CBD induced periosteal bone formation at higher rates than collagen sheet alone and bFGF. bFGF-PKD-CBD markedly enhances bone formation and has higher collagen-binding ability than bFGF-CBD in in vitro protein release assays	Hathewaya histolytica

Organism

Organism	UniProt	Comment	Textmining
Hathewaya histolytica	Q46085	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
class II collagenase	-	Hathewaya histolytica
Clostridium histolyticum class II collagenase	-	Hathewaya histolytica
Clostridium histolyticum collagenase	-	Hathewaya histolytica
ColH	-	Hathewaya histolytica

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Release 2023.1 (January 2023)