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Literature summary for 3.4.24.3 extracted from
- Mechanism of action of collagenase clostridium histolyticum for clinical application (2016), Eur. J. Clin. Pharmacol., 18, 263-272 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | mechanism of action of collagenase clostridium histolyticum for clinical application | Hathewaya histolytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Hathewaya histolytica | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the presence of Ca2+ alters the collagen-binding domain enzyme structure upon collagen binding from beta-sandwich to alpha-helix | Hathewaya histolytica |  |
| Zn2+ | a zinc metalloproteinase | Hathewaya histolytica | |
| Zn2+ | a zinc metalloproteinase, residues Glu446 and Glu451 of the S3 segment are responsible for the catalysis through interaction with the residues of His linked to Zn2+, forming two triads Glu446-His419-Zn2+ and Glu451-His415-Zn2+, which play a critical role in the catalytic mechanism | Hathewaya histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112021 | - |
sequence calculation, mature enzyme | Hathewaya histolytica |
| 113897 | - |
sequence calculation, mature enzyme | Hathewaya histolytica |
| 116375 | - |
sequence calculation, immature enzyme | Hathewaya histolytica |
| 126239 | - |
type beta bond, sequence calculation, immature enzyme | Hathewaya histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | Hathewaya histolytica | type II collagenase ColH acts inside of the collagen triple helix molecule | ? | - |
? | |
| Collagen type I + H2O | Hathewaya histolytica | type I collagenase ColG acts on the N-terminal level of the triple helix of collagen | ? | - |
? | |
| collagen type II + H2O | Hathewaya histolytica | - |
? | - |
? | |
| Collagen type III + H2O | Hathewaya histolytica | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | Q46085 | - |
- |
| Hathewaya histolytica | Q9X721 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | type II collagenase ColH acts inside of the collagen triple helix molecule | Hathewaya histolytica | ? | - |
? | |
| Collagen type I + H2O | type I collagenase ColG acts on the N-terminal level of the triple helix of collagen | Hathewaya histolytica | ? | - |
? | |
| collagen type II + H2O | - |
Hathewaya histolytica | ? | - |
? | |
| Collagen type III + H2O | - |
Hathewaya histolytica | ? | - |
? | |
| collagen type IV + H2O | ColG is active on collagen IV only in vitro. ColG promotes interaction with fibrils but not with the triple helix | Hathewaya histolytica | ? | - |
? | |
| additional information | ColH is active on the triple helix itself | Hathewaya histolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the S1 domain is the catalytic segment containing the sequence HEXXH, a group of amino acids that constitute the catalytic center. S2 is one extra domain, and segments S3a and S3b work together forming a union. Enzyme domain structure, overview | Hathewaya histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ColG | - |
Hathewaya histolytica |
| ColH | - |
Hathewaya histolytica |
| collagenase | - |
Hathewaya histolytica |
| collagenase clostridium histolyticum | - |
Hathewaya histolytica |
| type I collagenase | - |
Hathewaya histolytica |
| type II collagenase | - |
Hathewaya histolytica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | clases of collagenases, overview | Hathewaya histolytica |
| evolution | classes of collagenases, overview | Hathewaya histolytica |
| additional information | mechanism of action of collagenase clostridium histolyticum for clinical application. Residues Glu446 and Glu451 of the S3 segment are responsible for the catalysis through interaction with the residues of His linked to Zn2+, forming two triads Glu446-His419-Zn2+ and Glu451-His415-Zn2+, which play a critical role in the catalytic mechanism of ColH | Hathewaya histolytica |
| additional information | mechanism of action of collagenase clostridium histolyticum for clinical application. The action of ColG is done in intermediate areas of the collagen triple helix. Structure-function relationship, overview | Hathewaya histolytica |
| additional information | the S1 domain is the catalytic segment containing the sequence HEXXH, a group of amino acids that constitute the catalytic center. The S3 domain is the segment where the collagen-binding takes place. The S2a and S2b segments are the segments between domains. Enzyme domain structure, overview | Hathewaya histolytica |
| physiological function | the enzyme is involved in collagen degradation. Type I collagenase ColG acts on the N-terminal level of the triple helix of collagen. This first step is followed by the loosening of the C-terminal structure of the enzyme converting the collagen as a gelatinase and degradation of the interstitial collagen obtaining little oligopeptides | Hathewaya histolytica |
| physiological function | the enzyme is involved in collagen degradation. Type II collagenase ColH acts inside of the collagen triple helix molecule. This first step is followed by the loosening of the C-terminal structure of the enzyme converting the collagen as a gelatinase and degradation of the interstitial collagen obtaining little oligopeptides | Hathewaya histolytica |
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