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Literature summary for 3.4.24.3 extracted from
- Clostridium histolyticum collagenase. II. Partial characterization (1972), Enzymologia, 43, 45-55.
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes | Hathewaya histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Hathewaya histolytica |  |
| glutathione | reduced | Hathewaya histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.71 | - |
Carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala | - |
Hathewaya histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 147200 | - |
gel filtration | Hathewaya histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | Hathewaya histolytica | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hathewaya histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Azocoll + H2O | denatured collagen with bound dye, no hydrolysis | Hathewaya histolytica | Hydrolyzed azocoll | - |
? | |
| carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala + H2O | - |
Hathewaya histolytica | carbobenzoxy-Gly-Pro-Gly + Gly-Pro-Ala | - |
? | |
| Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form | Hathewaya histolytica | Hydrolyzed collagen | - |
? | |
| Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | Hathewaya histolytica | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hathewaya histolytica |
| 40 | - |
3 h, Ca2+ added | Hathewaya histolytica |
| 50 | - |
2 h, Ca2+ added | Hathewaya histolytica |
| 60 | - |
5 min, complete inactivation | Hathewaya histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.8 | - |
Hathewaya histolytica |
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Release 2023.1 (January 2023)
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