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Literature summary for 3.4.24.3 extracted from
- Expression and characterization of the prtV gene encoding a collagenase from Vibrio parahaemolyticus in Escherichia coli (1999), Microbiology, 145, 143-150.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Vibrio parahaemolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | activity restored by Co2+, Mn2+, Ca2+, Cu2+, Ni2+ and Zn2+ | Vibrio parahaemolyticus |  |
| 2-mercaptoethanol | - |
Vibrio parahaemolyticus | |
| EDTA | activity restored by Co2+, Mn2+, Ca2+, Cu2+, Ni2+ and Zn2+ | Vibrio parahaemolyticus | |
| EGTA | activity restored by Co2+, Mn2+, Ca2+, Cu2+, Ni2+ and Zn2+ | Vibrio parahaemolyticus | |
| Guanidine-HCl | - |
Vibrio parahaemolyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Vibrio parahaemolyticus | |
| 1.06 | - |
2-furanacryloyl-Leu-Gly-Pro-Ala | - |
Vibrio parahaemolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Vibrio parahaemolyticus |
| 64000 | - |
1 * 64000, SDS-PAGE | Vibrio parahaemolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | Vibrio parahaemolyticus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio parahaemolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Vibrio parahaemolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-furanacryloyl-Leu-Gly-Pro-Ala + H2O | - |
Vibrio parahaemolyticus | 2-furanacryloyl-Leu + Gly-Pro-Ala | - |
? | |
| Collagen + H2O | - |
Vibrio parahaemolyticus | Hydrolyzed collagen | - |
? | |
| Collagen + H2O | - |
Vibrio parahaemolyticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000, SDS-PAGE | Vibrio parahaemolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Vibrio parahaemolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Vibrio parahaemolyticus |
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