Information on EC 4.2.1.11 - phosphopyruvate hydratase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
4.2.1.11
-
RECOMMENDED NAME
GeneOntology No.
phosphopyruvate hydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-phospho-D-glycerate = phosphoenolpyruvate + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition
-
-
-
-
elimination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1-butanol autotrophic biosynthesis (engineered)
-
-
Bifidobacterium shunt
-
-
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Entner-Doudoroff pathway II (non-phosphorylative)
-
-
Entner-Doudoroff pathway III (semi-phosphorylative)
-
-
ethylene biosynthesis V (engineered)
-
-
formaldehyde assimilation I (serine pathway)
-
-
gluconeogenesis I
-
-
gluconeogenesis II (Methanobacterium thermoautotrophicum)
-
-
gluconeogenesis III
-
-
glycerol degradation to butanol
-
-
glycolysis
-
-
Glycolysis / Gluconeogenesis
-
-
glycolysis I (from glucose 6-phosphate)
-
-
glycolysis II (from fructose 6-phosphate)
-
-
glycolysis III (from glucose)
-
-
glycolysis IV (plant cytosol)
-
-
glycolysis V (Pyrococcus)
-
-
heterolactic fermentation
-
-
Metabolic pathways
-
-
Methane metabolism
-
-
Microbial metabolism in diverse environments
-
-
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
-
-
Rubisco shunt
-
-
superpathway of glucose and xylose degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
2-phospho-D-glycerate hydro-lyase (phosphoenolpyruvate-forming)
Also acts on 3-phospho-D-erythronate.
CAS REGISTRY NUMBER
COMMENTARY hide
9014-08-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 168
SwissProt
Manually annotated by BRENDA team
strain 168
SwissProt
Manually annotated by BRENDA team
strain BE1
Uniprot
Manually annotated by BRENDA team
strain BE1
Uniprot
Manually annotated by BRENDA team
-
TREMBL
Manually annotated by BRENDA team
strain BI07
UniProt
Manually annotated by BRENDA team
strain S16
-
-
Manually annotated by BRENDA team
strain S16
-
-
Manually annotated by BRENDA team
strain BBSF
-
-
Manually annotated by BRENDA team
strain BBSF
-
-
Manually annotated by BRENDA team
strain S123
UniProt
Manually annotated by BRENDA team
strain S123
UniProt
Manually annotated by BRENDA team
Borrelia burgdorferi
-
-
-
Manually annotated by BRENDA team
Borrelia burgdorferi B31-MI-16
-
-
-
Manually annotated by BRENDA team
strain strain BO5.10
-
-
Manually annotated by BRENDA team
strain 2308, 100% sequence identity with strain A19
UniProt
Manually annotated by BRENDA team
strain 2308, 100% sequence identity with strain A19
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene LOS2
UniProt
Manually annotated by BRENDA team
propagated in livers of male Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
enolase 1 and 2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isolated from sheep in Qinghai province of China
-
-
Manually annotated by BRENDA team
strain PaPt36
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain IP-1
-
-
Manually annotated by BRENDA team
strain IP-1
-
-
Manually annotated by BRENDA team
gram-positive bacteria, ATCC9790
SwissProt
Manually annotated by BRENDA team
strain HTA426
SwissProt
Manually annotated by BRENDA team
clone RRIM 600
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain AZV
SwissProt
Manually annotated by BRENDA team
serovar Lai
-
-
Manually annotated by BRENDA team
serovar Lai
-
-
Manually annotated by BRENDA team
strain B-512FMCM, constitutively secreting dextransucrase
SwissProt
Manually annotated by BRENDA team
strain B-512FMCM, constitutively secreting dextransucrase
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 18
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain NF54
-
-
Manually annotated by BRENDA team
strain 17XL
SwissProt
Manually annotated by BRENDA team
strain 17XL
SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
L. Cv. Baker 296
-
-
Manually annotated by BRENDA team
serovar Typhi Ty2
-
-
Manually annotated by BRENDA team
isolate not clearly defined
TREMBL
Manually annotated by BRENDA team
isolates SFCornell, SF-Daft, SF-Florida
TREMBL
Manually annotated by BRENDA team
isolates SN-UCD1, SN-37R, SN-MU1, SN-MU2, SN-MUCAT2, SN-OT1
TREMBL
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Staphylococcus aureus Wood 46
Wood 46
Uniprot
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain R36A
GenBank
Manually annotated by BRENDA team
FA-1
-
-
Manually annotated by BRENDA team
FA-1
-
-
Manually annotated by BRENDA team
strain 05ZYH33, serotype 2
UniProt
Manually annotated by BRENDA team
Streptomyces mutans
-
-
-
Manually annotated by BRENDA team
Streptomyces pneumoniae
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain ISS413
-
-
Manually annotated by BRENDA team
strain ISS413
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Xanthomonas oryzae pv. oryzae
UniProt
Manually annotated by BRENDA team
5 isoenzymes
-
-
Manually annotated by BRENDA team
3 isoelectric forms
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Z)-3-chloro-2-phosphoenolpyruvate + OH-
?
show the reaction diagram
-
wild-type enzyme catalyzes hydrolysis of (Z)-3-chloro-2-phosphoenolpyruvate by addition of OH- and elimination of Cl- at C-3
-
-
?
2,3-diketo-5-methylthiopentane 1-phosphate
2-hydroxy-3-keto-5-methylthiopent-1-ene 1-phosphate
show the reaction diagram
2,3-diketohexane 1-phosphate
?
show the reaction diagram
2,3-dioxo-5-methylthio-1-phosphopentane + 4 H+
3-hydroxy-5-methyl-thio-pent-2-en-1-yl-phosphate + H2O
show the reaction diagram
-
-
-
-
?
2-phospho-D-glycerate
?
show the reaction diagram
2-phospho-D-glycerate
phosphoenolpyruvate
show the reaction diagram
2-phospho-D-glycerate
phosphoenolpyruvate + H2O
show the reaction diagram
3-phospho-D-erythronate
?
show the reaction diagram
-
-
-
-
?
3-phospho-D-glycerate
?
show the reaction diagram
-
-
-
-
?
D-tartronate semialdehyde phosphate
?
show the reaction diagram
D-tartronate semialdehyde-2-phosphate
?
show the reaction diagram
-
substrate analogue that changes its spectrum while bound to the enzyme
-
?
phosphoenolpyruvate
2-phospho-D-glycerate
show the reaction diagram
phosphoenolpyruvate + H2O
2-phospho-D-glycerate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-diketo-5-methylthiopentane 1-phosphate
2-hydroxy-3-keto-5-methylthiopent-1-ene 1-phosphate
show the reaction diagram
2,3-dioxo-5-methylthio-1-phosphopentane + 4 H+
3-hydroxy-5-methyl-thio-pent-2-en-1-yl-phosphate + H2O
show the reaction diagram
-
-
-
-
?
2-phospho-D-glycerate
?
show the reaction diagram
2-phospho-D-glycerate
phosphoenolpyruvate
show the reaction diagram
-
-
-
-
r
2-phospho-D-glycerate
phosphoenolpyruvate + H2O
show the reaction diagram
phosphoenolpyruvate + H2O
2-phospho-D-glycerate
show the reaction diagram
-
analysis of pathogenesis of Bacillus anthracis: binding of human plasminogen and laminin
-
-
r
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Al3+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 68.8% of the maximal activity with 10 mM Mg2+
Cd2+
-
14% of the activation with Mg2+
Cu2+
-
20% of the activiation with Mg2+
KBr
dimeric form 10-20% activated, monomeric form strongly inhibited
Ni2+
can partially substitute for Mg2+ or Zn2+ at 10 mM showing 90.5% of the maximal activity with 10 mM Mg2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-fluoro-2-phosphonoacetohydroxamate
-
competitive inhibitor
2-phosphoglycerate
3-aminoenolpyruvate phosphate
-
-
-
3-hydroxy-2-nitro-1-phosphonopropane
-
-
3-hydroxypropionic acid phosphate
-
-
4-hydroxy-2-nonenal
acrolein
citrate
competitive
Cr2+
leads to complete inhibition at 10 mM
Cu2+
leads to complete inhibition at 10 mM
cumene hydroperoxide
-
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50C and pH 7 for 2 h
D-2,3-dihydroxyisobutyric acid 2-phosphate
-
-
D-erythro-2,3-dihydroxybutyric acid 2-phosphate
-
-
D-erythro-2,3-dihydroxybutyric acid 3-phosphate
-
-
D-glycerate-2-phosphate
-
mixed-type inhibition in the binding of D-glycerate-2-phosphate and D-phosphoglycerate mutase to the D-glycerate-2-phosphate binding site on the enolase in absence of D-glycerate-2,3-diphosphate, inhibition is almost fully reverted by D-glycerate-2,3-diphosphate
D-glyceric acid 3-phosphate
-
-
D-lactic acid phosphate
-
-
D-tartronate semialdehyde phosphate
-
-
diphosphate
-
inhibits natural enolase and recombinant protein
fluoride
Hg2+
leads to complete inhibition at 10 mM
hydrogen peroxide
-
inhibitory at 0.25%, at pH 7
iodoacetamide
-
binds to cysteine residues
KBr
monomeric form
KCl
monomeric form
methylglyoxal
monofluorophosphate
-
-
Na+
-
50% inhibition around 0.3-0.4 M
NaCl
inhibits dimeric and monomeric forms of the enzyme, inhibition stronger for the monomeric form
NaClO4
p19ras
-
when full-length p19ras and C-terminal region are bound to NSE, it inhibits the enzymatic activity of NSE, p19ras interacts with enolase alpha and represses its enzymatic activity in vitro
-
peracetic acid
-
1% residual activity after treatment with 4 mM peracetic acid at 25C and pH 7 for 15 min
phosphate
phosphonoacetohydroxamate
PO43-
mimics the phosphate group of substrate
SO42-
-
induces a complete closure of catalytic site loops
tert-butyl hydroperoxide
-
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50C and pH 7 for 3 h
trans-2-nonenal
additional information