Literature summary for 4.2.1.11 extracted from

Sharma, V.; Gupta, R.; Jhingran, A.; Singh, B.P.; Sridhara, S.; Gaur, S.N.; Arora, N.
Cloning, recombinant expression and activity studies of a major allergen 'enolase' from the fungus Curvularia lunata (2006), J. Clin. Immunol., 26, 360-369.

Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
isolated from cDNA library, expressed in Escherichia coli BL21, expression vector pET22b, 60% sequence similarities to human non-neuronal alpha enolase, conserved residues determined	Curvularia lunata

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	metal-binding	Curvularia lunata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	SDS-PAGE	Curvularia lunata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Curvularia lunata	-	phosphoenolpyruvate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Curvularia lunata	Q96VP4	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein amounts are 10-15% of the total protein, purification from inclusion bodies yields 0.5 mg per liter, allergenic activity of the recombinant protein tested by ELIZA	Curvularia lunata

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture medium	4 d old	Curvularia lunata	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	biological activity shown, enzyme activity comparable to those of Candida	Curvularia lunata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Curvularia lunata	phosphoenolpyruvate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
enolase	-	Curvularia lunata

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Release 2023.1 (January 2023)