Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS using the pRSETA expression vector, recombinant His-tagged protein | Leuconostoc mesenteroides |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, from preparations of purified dextransucrase | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
F- | non-competitive inhibition without phosphate and in presence of 1 mM phosphate, competitive inhibition in presence of 20 mM phosphate | Leuconostoc mesenteroides | |
additional information | recombinant enolase inhibits activity of purified dextransucrase | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.61 | - |
2-phospho-D-glycerate | at pH 6.8 | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47550 | - |
deduced from sequence | Leuconostoc mesenteroides |
51400 | - |
SDS-PAGE | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Leuconostoc mesenteroides | - |
phosphoenolpyruvate + H2O | - |
? | |
2-phospho-D-glycerate | Leuconostoc mesenteroides B-512FMCM | - |
phosphoenolpyruvate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | Q1ET73 | constitutively secreting dextransucrase | - |
Leuconostoc mesenteroides B-512FMCM | Q1ET73 | constitutively secreting dextransucrase | - |
Purification (Comment) | Organism |
---|---|
extracellular enolase identified from isolates of extracellular dextransucrase from cultures of Leuconostoc mesenteroides, accumulation as small crystals | Leuconostoc mesenteroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | strain B-512FMCM | Leuconostoc mesenteroides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of recombinant protein spectrophotometrically measured by conversion of 2-phospho-D-glycerate to phosphoenolpyruvate, substrate concentrations of 3 mM | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Leuconostoc mesenteroides | phosphoenolpyruvate + H2O | - |
? | |
2-phospho-D-glycerate | - |
Leuconostoc mesenteroides B-512FMCM | phosphoenolpyruvate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | extracellular enolase predicted as octamer, with two of its subunits defining the asymmetric unit of the crystal | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay at | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
optimum determined by activity assay | Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.3 | catalytic activity above and below pH 6.8 remains constant at 30% of maximal activity | Leuconostoc mesenteroides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
F- | in presence of 1 mM phosphate | Leuconostoc mesenteroides | |
5.4 | - |
F- | in absence of phosphate | Leuconostoc mesenteroides | |
9.2 | - |
F- | in presence of 20 mM phosphate | Leuconostoc mesenteroides |