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Literature summary for 4.2.1.11 extracted from

  • Pietkiewicz, J.; Gamian, A.; Staniszewska, M.; Danielewicz, R.
    Inhibition of human muscle-specific enolase by methylglyoxal and irreversible formation of advanced glycation end products (2009), J. Enzyme Inhib. Med. Chem., 24, 356-364.
    View publication on PubMed
Search for more literature for 4.2.1.11

General Stability

General Stability Organism
the enzyme maintains only 5% of the initial catalytic activity upon glycation for the first 90 min Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
methylglyoxal incubation of0.015 mM enzyme with 2 mM, 3.1 mM and 4.34 mM methylglyoxal in 100 mM phosphate buffer pH 7.4 for 3 h causes the loss a 32%, 55% and 82% of initial specific activity, respectively. Inhibition of enolase by methylglyoxal and formation of enolase-derived glycation products arises more effectively in slight alkaline conditions and in the presence of inorganic phosphate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 2-phospho-D-glycerate native enzyme, in the absence of methyglyoxal, in 50 mM imidazole-HCl buffer, pH 6.8, with 0.4 M KCl and 3 mM MgSO4 Homo sapiens
0.58
-
 phosphoenolpyruvate native enzyme, in the absence of methyglyoxal, in 50 mM imidazole-HCl buffer, pH 6.8, with 0.4 M KCl and 3 mM MgSO4 Homo sapiens
0.66
-
 2-phospho-D-glycerate glycated enzyme, in the presence of 40% (v/v) methyglyoxal, in 50 mM imidazole-HCl buffer, pH 6.8, with 0.4 M KCl and 3 mM MgSO4 Homo sapiens
0.83
-
 phosphoenolpyruvate glycated enzyme, in the presence of 40% (v/v) methyglyoxal, in 50 mM imidazole-HCl buffer, pH 6.8, with 0.4 M KCl and 3 mM MgSO4 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, the optimum concentration is 3 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Homo sapiens phosphoenolpyruvate + H2O
-
 r

Synonyms

Synonyms Comment Organism
2-phospho-D-glycerate hydrolyase
-
 Homo sapiens
enolase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Homo sapiens