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Literature summary for 4.2.1.11 extracted from

  • Sims, P.A.; Menefee, A.L.; Larsen, T.M.; Mansoorabadi, S.O.; Reed, G.H.
    Structure and catalytic properties of an engineered heterodimer of enolase composed of one active and one inactive subunit (2006), J. Mol. Biol., 355, 422-431.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
engineered K345A/N80D/N126D heterodimer in complex with substrate/product, 1.85A resolution, batch method Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
E211Q inactive, but properly folded Saccharomyces cerevisiae
K345A inactive Saccharomyces cerevisiae
K345A/N80D/N126D heterodimer with one inactive K345A subunit and one active N80D and N126D subunit Saccharomyces cerevisiae
N80D/N126D mutant with surface mutations to facilitate ion-exchange chromatographic separation Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 2-phospho-D-glycerate wild type, 25°C, Mg2+, pH 7.5 Saccharomyces cerevisiae
0.05
-
 2-phospho-D-glycerate K345A/N80D/N126D, 25°C, Mg2+, pH 7.5 Saccharomyces cerevisiae
0.057
-
 2-phospho-D-glycerate N80D/N126D, 25°C, Mg2+, pH 7.5 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Saccharomyces cerevisiae
-
 phosphoenolpyruvate + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00924
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 r

Subunits

Subunits Comment Organism
heterodimer engineered type Saccharomyces cerevisiae
homodimer N80D/N126D Saccharomyces cerevisiae