General Stability | Organism |
---|---|
Mg2+, 20% sucrose or glycerol stabilize enzyme in dilute solutions | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-phosphoglycerate | substrate inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Sus scrofa | |
0.05 | - |
2-phospho-D-glycerate | - |
Sus scrofa | |
0.11 | - |
phosphoenolpyruvate | - |
Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | required for maximal activity | Sus scrofa | |
Mg2+ | Km: 0.43 mM | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Sus scrofa |
88000 | - |
sedimentation velocity measurement | Sus scrofa |
110000 | - |
gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
4°C, 1 mg/ml or more, 0.1 M imidazole HCl, pH 7.0 and 2 mM MgCl2, stable for at least 2 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Sus scrofa | phosphoenolpyruvate | - |
r | |
2-phospho-D-glycerate | - |
Sus scrofa | phosphoenolpyruvate + H2O | - |
? | |
phosphoenolpyruvate | - |
Sus scrofa | 2-phospho-D-glycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Sus scrofa |