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Literature summary for 4.2.1.11 extracted from

  • Labbe, M.; Peroval, M.; Bourdieu, C.; Girard-Misguich, F.; Pery, P.
    Eimeria tenella enolase and pyruvate kinase: a likely role in glycolysis and in others functions (2006), Int. J. Parasitol., 36, 1443-1452.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
identified from cDNA library, expression in Escherichia coli Eimeria tenella

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm partly localized inside the nucleus of sporozoites and schizonts, partly secreted, apex of the first generation of merozoites, relocalization inside sporozoites observed Eimeria tenella 5737
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel electrophoresis Eimeria tenella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Eimeria tenella
-
phosphoenolpyruvate + H2O
-
r
2-phospho-D-glycerate Eimeria tenella PaPt36
-
phosphoenolpyruvate + H2O
-
r

Organism

Organism UniProt Comment Textmining
Eimeria tenella Q967Y8
-
-
Eimeria tenella PaPt36 Q967Y8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, pET22/14-X vector, His-tag purification, SDS-PAGE Eimeria tenella

Source Tissue

Source Tissue Comment Organism Textmining
schizont isolated after infection of 2-week-old White Leghorn PA12 chickens for 68 h, protein but not cDNA detected in the sporozoite stage, expression enhanced during the first schizogony Eimeria tenella
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
multifunctional role of enolase, participation in the parasitic invasion process and in the control of gene regulation Eimeria tenella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Eimeria tenella phosphoenolpyruvate + H2O
-
r
2-phospho-D-glycerate
-
Eimeria tenella PaPt36 phosphoenolpyruvate + H2O
-
r

Synonyms

Synonyms Comment Organism
enolase
-
Eimeria tenella