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Literature summary for 4.2.1.11 extracted from

  • Brewer, J.M.; Glover, C.V.; Holland, M.J.; Lebioda, L.
    Enzymatic function of loop movement in enolase: preparation and some properties of H159N, H159A, H159F, and N207A enolases (2003), J. Protein Chem., 22, 353-361.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H159A less than 1% of the activity compared to the wild type Saccharomyces cerevisiae
H159F less than 1% of the activity compared to the wild type Saccharomyces cerevisiae
H159N less than 1% of the activity compared to the wild type Saccharomyces cerevisiae
N207A 50% of the activity compared to the wild type Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibitor above 1 mM, N207A, H159A, H159N and H159F mutants are stimulated at this concentration Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
2-phospho-D-glycerate Km ranges between 0.047 mM and 0.130 mM between pH 6.0 and pH 8.6 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
gel filtration, mutant N207A enzyme Saccharomyces cerevisiae
96000
-
gel filtration, wild type enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
isozyme 1
-

Purification (Commentary)

Purification (Comment) Organism
wild type and several mutant recombinant enzymes using chromatography on Sepharose Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
H159N mutant, pH 7.8, 21ºC Saccharomyces cerevisiae
1.9
-
H159F mutant, pH 7.8, 21ºC Saccharomyces cerevisiae
3.3
-
H159A mutant, pH 7.8, 21ºC Saccharomyces cerevisiae
248
-
N207A mutant, pH 7.8, 21ºC Saccharomyces cerevisiae
442
-
wild type, pH 7.8, 21ºC Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Saccharomyces cerevisiae phosphoenolpyruvate
-
r
D-tartronate semialdehyde-2-phosphate substrate analogue that changes its spectrum while bound to the enzyme Saccharomyces cerevisiae ?
-
?

Synonyms

Synonyms Comment Organism
enolase
-
Saccharomyces cerevisiae