Protein Variants | Comment | Organism |
---|---|---|
H159A | less than 1% of the activity compared to the wild type | Saccharomyces cerevisiae |
H159F | less than 1% of the activity compared to the wild type | Saccharomyces cerevisiae |
H159N | less than 1% of the activity compared to the wild type | Saccharomyces cerevisiae |
N207A | 50% of the activity compared to the wild type | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | inhibitor above 1 mM, N207A, H159A, H159N and H159F mutants are stimulated at this concentration | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
2-phospho-D-glycerate | Km ranges between 0.047 mM and 0.130 mM between pH 6.0 and pH 8.6 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
gel filtration, mutant N207A enzyme | Saccharomyces cerevisiae |
96000 | - |
gel filtration, wild type enzyme | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
isozyme 1 | - |
Purification (Comment) | Organism |
---|---|
wild type and several mutant recombinant enzymes using chromatography on Sepharose | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
H159N mutant, pH 7.8, 21ºC | Saccharomyces cerevisiae |
1.9 | - |
H159F mutant, pH 7.8, 21ºC | Saccharomyces cerevisiae |
3.3 | - |
H159A mutant, pH 7.8, 21ºC | Saccharomyces cerevisiae |
248 | - |
N207A mutant, pH 7.8, 21ºC | Saccharomyces cerevisiae |
442 | - |
wild type, pH 7.8, 21ºC | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Saccharomyces cerevisiae | phosphoenolpyruvate | - |
r | |
D-tartronate semialdehyde-2-phosphate | substrate analogue that changes its spectrum while bound to the enzyme | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Saccharomyces cerevisiae |