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Literature summary for 4.2.1.11 extracted from
- Purification and properties of gammagamma-enolase from pig brain (1999), J. Protein Chem., 18, 103-115.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | noncompetitive with either 2-phosphoglycerate or Mg2+ | Sus scrofa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
2-phospho-D-glycerate | - |
Sus scrofa | |
| 0.25 | - |
phosphoenolpyruvate | - |
Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Km: 0.5 mM | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Sus scrofa |
| 85000 | - |
sucrose density gradient ultracentrifugation | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
3 enolase isoforms | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gamma,gamma-enolase | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
| muscle | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 82.2 | - |
- |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Sus scrofa | phosphoenolpyruvate | - |
r | |
| 2-phospho-D-glycerate | - |
Sus scrofa | phosphoenolpyruvate + H2O | - |
? | |
| phosphoenolpyruvate | - |
Sus scrofa | 2-phospho-D-glycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Sus scrofa |
| 7.4 | - |
liver enzyme, both directions | Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | about 55% of maximal activity at pH 6.8 and at pH 8.0 | Sus scrofa |
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