Cloned (Comment) | Organism |
---|---|
cDNA inserted in PGEX-4T-3 expressed in Escherichia coli, glutathione S-transferase fusion protein, protein with 436 amino acid residues, 70-80% sequence similarity to enolases from other organisms, including helminth parasites | Anisakis simplex |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Anisakis simplex | - |
phosphoenolpyruvate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anisakis simplex | Q8MU59 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose-4B chromatography | Anisakis simplex |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | L3 state | Anisakis simplex | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
functional activity of recombinant protein shown, immunogenicity determined by ELISA and Western Blot using different sera, natural infection of humans by Anisakis simplex larvae lacks sufficient antigenic stimuli | Anisakis simplex |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Anisakis simplex | phosphoenolpyruvate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Anisakis simplex |