Literature summary for 4.2.1.11 extracted from

Raghunathan, K.; Harris, P.T.; Spurbeck, R.R.; Arvidson, C.G.; Arvidson, D.N.
Crystal structure of an efficacious gonococcal adherence inhibitor: An enolase from Lactobacillus gasseri (2014), FEBS Lett., 588, 2212-2216.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Lactobacillus gasseri

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 20% (w/v) PEG 550, 100 mM bicine pH 9.0, 100 mM sodium chloride	Lactobacillus gasseri

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	each monomer is binding a single Mg2+ cofactor	Lactobacillus gasseri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Lactobacillus gasseri	-	phosphoenolpyruvate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus gasseri	Q042F4	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography	Lactobacillus gasseri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Lactobacillus gasseri	phosphoenolpyruvate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
enolase	-	Lactobacillus gasseri

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Release 2023.1 (January 2023)