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Literature summary for 4.2.1.11 extracted from

  • Agarwal, S.; Kulshreshtha, P.; Bambah Mukku, D.; Bhatnagar, R.
    alpha-Enolase binds to human plasminogen on the surface of Bacillus anthracis (2008), Biochim. Biophys. Acta, 1784, 986-994.
    View publication on PubMed
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 phosphoenolpyruvate recombinant protein Bacillus anthracis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Bacillus anthracis 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 SDS-PAGE Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + H2O Bacillus anthracis analysis of pathogenesis of Bacillus anthracis: binding of human plasminogen and laminin 2-phospho-D-glycerate
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus anthracis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Vmax at 37°C and pH 6.8: 0.506 micromol/min/mg for the recombinant protein Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + H2O analysis of pathogenesis of Bacillus anthracis: binding of human plasminogen and laminin Bacillus anthracis 2-phospho-D-glycerate
-
 r

Synonyms

Synonyms Comment Organism
alpha-enolase
-
 Bacillus anthracis
rBaEn recombinant protein expressed in Escherichia coli with a 6×-His tag Bacillus anthracis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 at pH 6.8, recombinant protein Bacillus anthracis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 at pH 6.8, recombinant protein Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 at 37°C, recombinant protein Bacillus anthracis