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Literature summary for 4.2.1.11 extracted from

  • Nakagawa, T.; Nagayama, F.
    Enzymatic properties of enolase from fish muscle (1991), Comp. Biochem. Physiol. B, 98, 355-359.
No PubMed abstract available
Search for more literature for 4.2.1.11

Inhibitors

Inhibitors Comment Organism Structure
phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Cyprinus carpio
phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Oryctolagus cuniculus
phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Pagrus major
phosphate competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate Scomber japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 2-phospho-D-glycerate
-
 Scomber japonicus
0.05
-
 2-phospho-D-glycerate
-
 Pagrus major
0.061
-
 2-phospho-D-glycerate
-
 Oryctolagus cuniculus
0.069
-
 2-phospho-D-glycerate
-
 Cyprinus carpio
0.11
-
 phosphoenolpyruvate
-
 Scomber japonicus
0.2
-
 phosphoenolpyruvate
-
 Pagrus major
0.2
-
 phosphoenolpyruvate
-
 Cyprinus carpio
0.25
-
 phosphoenolpyruvate
-
 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 0.7 mM Pagrus major
Mg2+ Km: 0.67 mM Cyprinus carpio
Mg2+ Km: 0.73 mM Scomber japonicus
Mg2+ Km: 0.69 mM Oryctolagus cuniculus
Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Oryctolagus cuniculus
Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Pagrus major
Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Scomber japonicus
Mg2+ absolute requirement for divalent metal ions, Mg2+ is most effective Cyprinus carpio
Mn2+ can partially replace Mg2+ in activation Oryctolagus cuniculus
Mn2+ can partially replace Mg2+ in activation Pagrus major
Mn2+ can partially replace Mg2+ in activation Scomber japonicus
Mn2+ can partially replace Mg2+ in activation Cyprinus carpio
Zn2+ can partially replace Mg2+ in activation Oryctolagus cuniculus
Zn2+ can partially replace Mg2+ in activation Pagrus major
Zn2+ can partially replace Mg2+ in activation Scomber japonicus
Zn2+ can partially replace Mg2+ in activation Cyprinus carpio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Oryctolagus cuniculus
50000
-
 2 * 50000, SDS-PAGE Scomber japonicus
51000
-
 2 * 51000, SDS-PAGE Cyprinus carpio
52000
-
 2 * 52000, SDS-PAGE Pagrus major
92000
-
 gel filtration Oryctolagus cuniculus
93000
-
 gel filtration Scomber japonicus
94000
-
 gel filtration Cyprinus carpio
95500
-
 gel filtration Pagrus major

Organism

Organism UniProt Comment Textmining
Cyprinus carpio
-
-
-
Oryctolagus cuniculus
-
-
-
Pagrus major
-
-
-
Scomber japonicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-
muscle
-
 Pagrus major
-
muscle
-
 Scomber japonicus
-
muscle
-
 Cyprinus carpio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56.1
-
-
 Scomber japonicus
61.7
-
-
 Scomber japonicus
76.2
-
-
 Pagrus major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Oryctolagus cuniculus phosphoenolpyruvate
-
 r
2-phospho-D-glycerate
-
 Pagrus major phosphoenolpyruvate
-
 r
2-phospho-D-glycerate
-
 Scomber japonicus phosphoenolpyruvate
-
 r
2-phospho-D-glycerate
-
 Cyprinus carpio phosphoenolpyruvate
-
 r
2-phospho-D-glycerate
-
 Oryctolagus cuniculus phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate
-
 Pagrus major phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate
-
 Scomber japonicus phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate
-
 Cyprinus carpio phosphoenolpyruvate + H2O
-
 ?
phosphoenolpyruvate
-
 Oryctolagus cuniculus 2-phospho-D-glycerate
-
 r
phosphoenolpyruvate
-
 Pagrus major 2-phospho-D-glycerate
-
 r
phosphoenolpyruvate
-
 Scomber japonicus 2-phospho-D-glycerate
-
 r
phosphoenolpyruvate
-
 Cyprinus carpio 2-phospho-D-glycerate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Scomber japonicus
dimer 2 * 51000, SDS-PAGE Cyprinus carpio
dimer 2 * 48000, SDS-PAGE Oryctolagus cuniculus
dimer 2 * 52000, SDS-PAGE Pagrus major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 formation of 2-phosphoglycerate Scomber japonicus
6.6
-
 formation of 2-phosphoglycerate Cyprinus carpio
6.9
-
 formation of phosphoenolpyruvate Oryctolagus cuniculus
6.9
-
 formation of phosphoenolpyruvate Cyprinus carpio
7
-
-
 Oryctolagus cuniculus
7.1
-
 formation of 2-phosphoglycerate Pagrus major
7.1
-
 formation of phosphoenolpyruvate Scomber japonicus
7.3
-
 formation of phosphoenolpyruvate Pagrus major