Cloned (Comment) | Organism |
---|---|
isolated from a genomic library, over-expressed in Escherichia coli, expression vector pET28a | Leishmania mexicana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | inhibits natural enolase and recombinant protein | Leishmania mexicana | |
Mg2+ | above 2 mM | Leishmania mexicana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
2-phospho-D-glycerate | recombinant protein | Leishmania mexicana | |
0.08 | - |
2-phospho-D-glycerate | natural enolase | Leishmania mexicana | |
0.2 | - |
phosphoenolpyruvate | recombinant protein | Leishmania mexicana | |
0.21 | - |
phosphoenolpyruvate | natural enolase | Leishmania mexicana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | enzyme activity only in cytosolic fraction, inactive form detected in the microsomal fraction, membrane-association and localization at the external face of the plasma membrane determined by digitonin treatment and immunofluorescence studies | Leishmania mexicana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity depends on, optimal concentration for activity between 1 mM and 2 mM | Leishmania mexicana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Leishmania mexicana | - |
phosphoenolpyruvate + H2O | - |
r | |
2-phospho-D-glycerate | Leishmania mexicana AZV | - |
phosphoenolpyruvate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | Q3HL75 | - |
- |
Leishmania mexicana AZV | Q3HL75 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein and natural enolase | Leishmania mexicana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania mexicana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enolase activity measured spectrophotometrically by following the change in phosphoenolpyruvate concentration, substrate concentrations up to 0.3 mM for 2-phospho-D-glycerate and up to 2 mM for phosphoenolpyruvate | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Leishmania mexicana | phosphoenolpyruvate + H2O | - |
r | |
2-phospho-D-glycerate | - |
Leishmania mexicana AZV | phosphoenolpyruvate + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Leishmania mexicana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay at | Leishmania mexicana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay at | Leishmania mexicana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.127 | - |
diphosphate | recombinant protein, substrate 2-phospho-D-glycerate | Leishmania mexicana | |
0.232 | - |
diphosphate | natural enolase, substrate 2-phospho-D-glycerate | Leishmania mexicana | |
0.238 | - |
diphosphate | natural enolase, substrate phosphoenolpyruvate | Leishmania mexicana | |
0.26 | - |
diphosphate | recombinant protein, substrate 2-phospho-D-glycerate | Leishmania mexicana |