Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | competitive | Pyrococcus furiosus | |
EDTA | 1 mM | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
2-phospho-D-glycerate | pH 8, 70°C | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM | Pyrococcus furiosus | |
Mn2+ | metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000 | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Pyrococcus furiosus | the enzyme probably functions in sugar fermentation pathway | phosphoenolpyruvate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q9UWJ5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.8 | - |
pH 8, 70°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Pyrococcus furiosus | phosphoenolpyruvate + H2O | - |
? | |
2-phospho-D-glycerate | the enzyme probably functions in sugar fermentation pathway | Pyrococcus furiosus | phosphoenolpyruvate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 46000 | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | no activity at 25°C, activity increases from 40°C to 90°C, the highest assay temperature used | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
88 | - |
t1/2: 300 min | Pyrococcus furiosus |
100 | - |
40 min, 50% inactivation. The half-life declines by a factor of about three in the presence of 700 mM KCl | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
- |
Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
citrate | pH 8, 70°C | Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme probably functions in sugar fermentation pathway | Pyrococcus furiosus |