Information on EC 2.7.1.11 - 6-phosphofructokinase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.1.11
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RECOMMENDED NAME
GeneOntology No.
6-phosphofructokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1,3-propanediol biosynthesis (engineered)
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Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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formaldehyde assimilation II (RuMP Cycle)
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Fructose and mannose metabolism
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Galactose metabolism
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glycolysis
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Glycolysis / Gluconeogenesis
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glycolysis I (from glucose 6-phosphate)
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glycolysis II (from fructose 6-phosphate)
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glycolysis III (from glucose)
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glycolysis IV (plant cytosol)
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Metabolic pathways
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Methane metabolism
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Microbial metabolism in diverse environments
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Pentose phosphate pathway
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-fructose-6-phosphate 1-phosphotransferase
D-Tagatose 6-phosphate and sedoheptulose 7-phosphate can act as acceptors. UTP, CTP and ITP can act as donors. Not identical with EC 2.7.1.105 6-phosphofructo-2-kinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-80-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Aeropyrum pernix DSM 11879
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UniProt
Manually annotated by BRENDA team
methylotrophic bacterium
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Arthrobacter nicotianae
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-
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Manually annotated by BRENDA team
strain B60
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Manually annotated by BRENDA team
e.g. Bacillus cereus, Bacillus megaterium, Bacillus mycoides, Bacillus subtilis 168M
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Manually annotated by BRENDA team
bumblebee
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-
Manually annotated by BRENDA team
Brussel's sprout
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-
Manually annotated by BRENDA team
blowfly
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-
Manually annotated by BRENDA team
camel
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
green alga
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Manually annotated by BRENDA team
Macf, cv. Marsh, grapefruit
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Manually annotated by BRENDA team
oyster
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
Dunaliella marina
green alga
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Manually annotated by BRENDA team
strain H200
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Manually annotated by BRENDA team
strain H200
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Manually annotated by BRENDA team
cod
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Manually annotated by BRENDA team
jerboa, euthermic and hibernating
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Manually annotated by BRENDA team
strain C10
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Manually annotated by BRENDA team
locust
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Manually annotated by BRENDA team
gypsy moth
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-
Manually annotated by BRENDA team
green alga
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain M1
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Manually annotated by BRENDA team
strain M1
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Manually annotated by BRENDA team
pea
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Manually annotated by BRENDA team
european flounder
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Manually annotated by BRENDA team
frog
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain CBS1057, PFK-1
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
wild potato species
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Manually annotated by BRENDA team
tomato, var. Eurocross BB
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Manually annotated by BRENDA team
potato, cv. Record, Hertha or Cara, isoforms I-IV
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Manually annotated by BRENDA team
protozoan, phenoset A
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Manually annotated by BRENDA team
freshwater turtle
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Manually annotated by BRENDA team
wheat
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Manually annotated by BRENDA team
Schizotrypanum
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Manually annotated by BRENDA team
i.e. Vigna mungo, black gram
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Manually annotated by BRENDA team
frog
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Manually annotated by BRENDA team
maize
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,N6-etheno-ATP + D-fructose 6-phosphate
1,N6-etheno-ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
1-carboxymethyl-ATP + D-fructose 6-phosphate
1-carboxymethyl-ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
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90% of activity with with ATP
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?
2-amino-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate
2-amino-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
6-mercapto-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate
6-mercapto-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate
show the reaction diagram
ATP + adenosine
ADP + ?
show the reaction diagram
-
-
-
?
ATP + D-fructose 1-phosphate
ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
ATP + D-fructose 6-phosphate
ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
ATP + D-tagatose 6-phosphate
ADP + ?
show the reaction diagram
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poor substrate for isoenzyme PFK2
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?
ATP + fructose
ADP + fructose 1-phosphate
show the reaction diagram
-
-
?
ATP + fructose 1-phosphate
ADP + fructose 1,6-bisphosphate
show the reaction diagram
ATP + glucose 6-phosphate
ADP + glucose 1,6-bisphosphate
show the reaction diagram
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-
?
ATP + ribose
ADP + ribose 1-phosphate
show the reaction diagram
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-
?
ATP + ribose 5-phosphate
ADP + ribose 1,5-bisphosphate
show the reaction diagram
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-
?
CTP + D-fructose 6-phosphate
CDP + D-fructose 1,6-bisphosphate
show the reaction diagram
dATP + D-fructose 6-phosphate
dADP + D-fructose 1,6-bisphosphate
show the reaction diagram
gamma-thio-ATP + fructose 6-phosphate
ADP + fructose 1-thio-phosphate-6-phosphate
show the reaction diagram
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3000fold lower kcat than with ATP
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?
GTP + D-fructose 6-phosphate
GDP + D-fructose 1,6-bisphosphate
show the reaction diagram
ITP + D-fructose 6-phosphate
IDP + D-fructose 1,6-bisphosphate
show the reaction diagram
TTP + D-fructose 6-phosphate
TDP + D-fructose 1,6-bisphosphate
show the reaction diagram
UTP + D-fructose 6-phosphate
UDP + D-fructose 1,6-bisphosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose 1-phosphate
ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
-
key enzyme of glucose metabolism
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-
?
ATP + D-fructose 6-phosphate
ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
optimal activity between 50 and 100 mM. 85% of maximal activity when the KCl concentration is 500 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-Hydroxycitrate
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(NH4)2SO4
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inhibits above 50 mM
1,3-diphosphoglycerate
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0.385 mM and 0.005 mM, 50% inhibition of PFK I and PFK II respectively, phosphate relieves from inhibition
2',3'-dialdehyde ATP
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irreversibly modifies a single lysine residue in the ATP-inhibitory site, thereby locking PFK in a permanantly inhibited state without affecting the integrity of the active site
2,3-diphosphoglycerate
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2-oxoglutarate
2-phosphoglycerate
3-phosphoglycerate
Agaric acid
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Aldolase
ammonium sulfate
50 mM, 50% inhibition
Antibodies against rabbit muscle enzyme
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not rabbit erythrocyte, leukocyte or platelet enzyme
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Arachidonoyl-CoA
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arginine phosphate
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ascorbate
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inhibition by ascorbate is PFK-1 concentration dependent. Ascorbate does not inhibit above 200 nM PFK-1. It is concluded that ascorbate inhibits PFK-1 dimers (and perhaps monomers) but not PFK-1 tetramers
aurintricarboxylic acid
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0.0002 mM, 50% inhibition at pH 7.3, reversed by addition of allosteric activators, i.e., fructose 2,6-bisphosphate or AMP, no inhibition at pH 8.0
cis-aconitate
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brain, not heart
citrate
clotrimazole
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clotrimazole alone induces dimerization of the enzyme reducing the population of tetramers, which is not observed when calmodulin is also present. Since PFK dimers are less active than tetramers, this can explain the inhibitory effect of clotrimazole
D-fructose 1,6-bisphosphate
D-Fructose 1-phosphate
D-fructose 6-phosphate
inhibition occurs at low concentrations of fructose 6-phosphate
D-Glucose 1,6-bisphosphate
D-glucose 6-phosphate
Dunaliella marina
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weak inhibition
diphosphate
Dithionitrobenzoic acid
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0.06 mM, 80% inhibition of PFK III, reversed by 2-mercaptoethanol, dithiothreitol or reduced glutathione
isocitrate
Lactate dehydrogenase
Li2CO3
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about 75% residual activity at 20 mM, about 40% residual activity at 40 mM, about 10% residual activity at 100 mM, less than 3% residual activity at 200 mM
Li2SO4
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about 55% residual activity at 100 mM, about 30% residual activity at 200 mM
linoleoyl-CoA
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-
lithium acetate
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about 80% residual activity in the presence of 0.009 mM lithium acetate
malate
Maleic anhydride
MgATP2-
NaCl
1 M, 50% inhibition
nucleoside triphosphate
oleoyl-CoA
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p-Chloromercurisulfonic acid
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0.025 mM, 80% inhibition of PFK III, reversed by 2-mercaptoethanol, dithiothreitol or reduced glutathione
palmitoyl-CoA
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low micromolar inhibitor, MgAMP and MgADP but not MgATP protect the enzyme against inhibition by palmitoyl-CoA. Acyl-protein thioesterase-1 reverses palmitoyl-CoA-mediated enzyme inhibition
palmitoylcarnitine-CoA
-
-
phosphatase
phosphate
phosphoenolpyruvate
Phosphoglycolate
Protein factor
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19000 Da protein promotes Zn2+ or Fe2+-dependent dissociation into inactive protomers, maximal inactivation at 0.001-0.02 mM Zn2+, inactivation is abolished at higher Zn2+ concentrations, Ca2+, Mg2+, Mn2+ can substitute for Zn2+ or Fe2+ only at millimolar concentrations, potency in descending order: Mn2+, Mg2+, Ca2+, inactivation can be reversed by the addition of ATP, fructose 1,6-bisphosphate, or fructose 2,6-bisphosphate
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pyridoxal 5'-phosphate
S-hexadecyl-CoA
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-
succinate
Succinic anhydride
Tris-HCl buffer
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additional information