Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | activation | Clostridium pasteurianum | |
ADP | positive effector | Clostridium pasteurianum | |
NH4+ | required for activity | Clostridium pasteurianum | |
NH4+ | can replace K+ | Clostridium pasteurianum |
Crystallization (Comment) | Organism |
---|---|
- |
Clostridium pasteurianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by ATP | Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
ATP | pH 8.0, 28°C | Clostridium pasteurianum | |
0.18 | - |
NH4+ | pH 8.0, 28°C | Clostridium pasteurianum | |
20 | - |
K+ | pH 8.0, 28°C | Clostridium pasteurianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required for activity, inhibition at high concentrations is reversed by increased concentrations of ATP | Clostridium pasteurianum | |
K+ | can replace NH4+ | Clostridium pasteurianum | |
Mg2+ | required for activity | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, high speed sedimentation equilibrium centrifugation or PAGE in the presence of 8 M urea or 7 M guanidine | Clostridium pasteurianum |
144000 | - |
high speed sedimentation equilibrium analysis | Clostridium pasteurianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat treatment, isopropanol, ammonium sulfate, DEAE-cellulose, crystallization | Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
- |
Clostridium pasteurianum |
Storage Stability | Organism |
---|---|
2°C, crystalline, in 45% saturated ammonium sulfate, at least 2 months | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | IDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | UDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, high speed sedimentation equilibrium centrifugation or PAGE in the presence of 8 M urea or 7 M guanidine | Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | - |
Clostridium pasteurianum |