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Literature summary for 2.7.1.11 extracted from

  • Uyeda, K.; Kurooka, S.
    Phosphofructokinase from Clostridium pasteurianum (1975), Methods Enzymol., 42C, 86-91.
No PubMed abstract available
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
ADP activation Clostridium pasteurianum
ADP positive effector Clostridium pasteurianum
NH4+ required for activity Clostridium pasteurianum
NH4+ can replace K+ Clostridium pasteurianum

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Clostridium pasteurianum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by ATP Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 ATP pH 8.0, 28°C Clostridium pasteurianum
0.18
-
 NH4+ pH 8.0, 28°C Clostridium pasteurianum
20
-
 K+ pH 8.0, 28°C Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required for activity, inhibition at high concentrations is reversed by increased concentrations of ATP Clostridium pasteurianum
K+ can replace NH4+ Clostridium pasteurianum
Mg2+ required for activity Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 4 * 35000, high speed sedimentation equilibrium centrifugation or PAGE in the presence of 8 M urea or 7 M guanidine Clostridium pasteurianum
144000
-
 high speed sedimentation equilibrium analysis Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment, isopropanol, ammonium sulfate, DEAE-cellulose, crystallization Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
-
 Clostridium pasteurianum

Storage Stability

Storage Stability Organism
2°C, crystalline, in 45% saturated ammonium sulfate, at least 2 months Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Clostridium pasteurianum ADP + D-fructose 1,6-bisphosphate
-
 ?
CTP + D-fructose 6-phosphate
-
 Clostridium pasteurianum CDP + D-fructose 1,6-bisphosphate
-
 ?
GTP + D-fructose 6-phosphate
-
 Clostridium pasteurianum GDP + D-fructose 1,6-bisphosphate
-
 ?
ITP + D-fructose 6-phosphate
-
 Clostridium pasteurianum IDP + D-fructose 1,6-bisphosphate
-
 ?
UTP + D-fructose 6-phosphate
-
 Clostridium pasteurianum UDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 35000, high speed sedimentation equilibrium centrifugation or PAGE in the presence of 8 M urea or 7 M guanidine Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.2
-
 Clostridium pasteurianum