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Literature summary for 2.7.1.11 extracted from

  • Hausler, R.E.; Holtum, J.A.M.; Latzko, E.
    Cytosolic phosphofructokinase from spinach leaves: 1. Purification and regulation by magnesium and phosphate (1989), Plant Physiol., 90, 1498-1505.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
citrate stimulation, by chelating of free Mg2+ Spinacia oleracea
Cl- 200 mM, 3.1fold activation of cytosolic PFK Spinacia oleracea
HAsO42- 50 mM, 2.9fold activation of cytosolic PFK Spinacia oleracea
HCO3- 200 mM, 2.1fold activation of cytosolic PFK Spinacia oleracea
NH4+ cytosolic isozyme is not activated Spinacia oleracea
NO3- 50 mM 1.6fold activation of cytosolic PFK Spinacia oleracea
phosphate 25 mM, 3.2fold activation of cytosolic PFK Spinacia oleracea
SO42- 50 mM, 2.6fold activation of cytosolic PFK Spinacia oleracea

General Stability

General Stability Organism
ATP stabilizes Spinacia oleracea
glycerol prevents inactivation of dilute cytosolic enzyme solution Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
2-phosphoglycerate 1.3 mM, 50% inhibition of cytosolic PFK, no inhibition in the presence of 5 or 25 mM phosphate Spinacia oleracea
diphosphate
-
 Spinacia oleracea
K+
-
 Spinacia oleracea
additional information not inhibited by 3-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by Na+ Spinacia oleracea
phosphoenolpyruvate 0.22 mM, 50% inhibition of cytosolic PFK in the presence of 1 mM fructose 6-phosphate, phosphate relives inhibition Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-
cytosol cytosolic and plastidic isoenzyme Spinacia oleracea 5829
-
plastid chloroplasts Spinacia oleracea 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Spinacia oleracea
Mg2+ MgATP is the active substrate Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic PFK, ammonium sulfate, DEAE-Sephacel, Blue Sepharose, ATP-agarose Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.5
-
 cytosolic PFK Spinacia oleracea

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tricine-NaOH buffer, pH 7.9, 50% v/v glycerol, at least 2 months Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Spinacia oleracea ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH-dependence is influenced by Mg2+ and/or phosphate Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.41
-
 diphosphate pH 7.7, 25°C Spinacia oleracea