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Literature summary for 2.7.1.11 extracted from

  • Ronimus, R.S.; Kawarabayasi, Y.; Kikuchi, H.; Morgan, H.W.
    Cloning, expression and characterisation of a Family B ATP-dependent phosphofructokinase activity from the hyperthermophilic crenarachaeon Aeropyrum pernix (2001), FEMS Microbiol. Lett., 202, 85-90.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

Inhibitors

Inhibitors Comment Organism Structure
ADP 1 mM, 82% inhibition Aeropyrum pernix
Cu2+ 1 mM, 52.4% inhibition Aeropyrum pernix
diphosphate 1 mM, 21% inhibition Aeropyrum pernix
GDP 1 mM, 46% inhibition Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
ATP pH 6.5, 95°C, discontinuous assay Aeropyrum pernix
1.212
-
D-fructose 6-phosphate pH 6.5, 95°C, discontinuous assay Aeropyrum pernix

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal activity between 50 and 100 mM. 85% of maximal activity when the KCl concentration is 500 mM Aeropyrum pernix
Mg2+ the optimal MgCl2 concentration for activity is 20-30 mM and the enzyme possesses 6, 36 and 74% activity compared to the maximal activity when the MgCl2 concentrations are 1.25, 2.5 and 5.0 mM, respectively Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YG89
-
-
Aeropyrum pernix DSM 11879 Q9YG89
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Aeropyrum pernix ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate
-
Aeropyrum pernix DSM 11879 ADP + D-fructose 1,6-bisphosphate
-
?
CTP + D-fructose 6-phosphate 20.6% of the activity with ATP Aeropyrum pernix CDP + D-fructose 1,6-bisphosphate
-
?
CTP + D-fructose 6-phosphate 20.6% of the activity with ATP Aeropyrum pernix DSM 11879 CDP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 6-phosphate 84.1% of the activity with ATP Aeropyrum pernix GDP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 6-phosphate 84.1% of the activity with ATP Aeropyrum pernix DSM 11879 GDP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Aeropyrum pernix

Synonyms

Synonyms Comment Organism
APE0012
-
Aeropyrum pernix
ATP-PFK
-
Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
assay at Aeropyrum pernix

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
85 95 85°C: 50% of maximal activity, 95°C: optimum Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
half-life: 30 min Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
Aeropyrum pernix

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 the pH optimum for activity of the enzyme is analysed only between the pH values of 5.5 to 7.5 due to concerns about instability of the ATP at more acid pH values and is found to be maximal between 5.5 and 6.5, but possesses 69% of maximal activity at pH 7.5 Aeropyrum pernix