Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 1 mM, 82% inhibition | Aeropyrum pernix | |
Cu2+ | 1 mM, 52.4% inhibition | Aeropyrum pernix | |
diphosphate | 1 mM, 21% inhibition | Aeropyrum pernix | |
GDP | 1 mM, 46% inhibition | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
ATP | pH 6.5, 95°C, discontinuous assay | Aeropyrum pernix | |
1.212 | - |
D-fructose 6-phosphate | pH 6.5, 95°C, discontinuous assay | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal activity between 50 and 100 mM. 85% of maximal activity when the KCl concentration is 500 mM | Aeropyrum pernix | |
Mg2+ | the optimal MgCl2 concentration for activity is 20-30 mM and the enzyme possesses 6, 36 and 74% activity compared to the maximal activity when the MgCl2 concentrations are 1.25, 2.5 and 5.0 mM, respectively | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YG89 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YG89 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Aeropyrum pernix | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Aeropyrum pernix DSM 11879 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | 20.6% of the activity with ATP | Aeropyrum pernix | CDP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | 20.6% of the activity with ATP | Aeropyrum pernix DSM 11879 | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | 84.1% of the activity with ATP | Aeropyrum pernix | GDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | 84.1% of the activity with ATP | Aeropyrum pernix DSM 11879 | GDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
APE0012 | - |
Aeropyrum pernix |
ATP-PFK | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
assay at | Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 95 | 85°C: 50% of maximal activity, 95°C: optimum | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life: 30 min | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | - |
Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | the pH optimum for activity of the enzyme is analysed only between the pH values of 5.5 to 7.5 due to concerns about instability of the ATP at more acid pH values and is found to be maximal between 5.5 and 6.5, but possesses 69% of maximal activity at pH 7.5 | Aeropyrum pernix |