Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | above 1 mM at 0.33 mM fructose 6-phosphate and 2 mM Mg2+, inhibition at a Mg/ATP ratio below 2; ADP partially reverses inhibition; fructose 6-phosphate partially reverses inhibition; Mg2+ partially reverses inhibition | Klebsiella aerogenes | |
citrate | cAMP, ADP or fructose 1,6-bisphosphate restore activity | Klebsiella aerogenes | |
CTP | - |
Klebsiella aerogenes | |
GTP | - |
Klebsiella aerogenes | |
additional information | not inhibited by ADP | Klebsiella aerogenes | |
nucleoside triphosphate | at a Mg/NTP ratio below 2 | Klebsiella aerogenes | |
UTP | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 2/1 | Klebsiella aerogenes | |
0.6 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 1.4/1 | Klebsiella aerogenes | |
1.4 | - |
D-fructose 6-phosphate | pH 8.0, 25, at a Mg2+/ATP ratio of 0.7/1 | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
strains PRL-R3, wild-type, and its mutant DD31 | - |
Purification (Comment) | Organism |
---|---|
protamine sulfate, ammonium sulfate, Sephadex G-200, partial purification | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.75 | - |
- |
Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | approx. 80% of maximal activity at pH 7 and pH 9, approx. half-maximal activity at pH 6.7 | Klebsiella aerogenes |