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Literature summary for 2.7.1.11 extracted from

  • Shirakihara, Y.; Evans, P.R.
    Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products (1988), J. Mol. Biol., 204, 973-994.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Geobacillus stearothermophilus
concentrated PFK solution is mixed with solutions containing fructose 6-phosphate, ADP and 2-methyl-2,4-pentanediol in a glass tube to a final concentration of 4 mg/ml PFK, 50% 2-methyl-2,4-pentanediol, 0.2 mM fructose 6-phosphate, 5 or 10 mM ATP, 100 mM Tris-HCl, pH 8.3, 5 mM MgCl2, 2.4 mM dithiothreitol, and 2.4 mM EDTA, crystal structure of PFK complexed with fructose 1,6-bisphosphate, ATP/Mg2+ and ADP/Mg2+ at 2.4 A resolution Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Escherichia coli
Mg2+ required for activity Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
containing overproducing plasmid pHE1007
-
Escherichia coli
-
containing overproducing plasmid pHE1012
-
Escherichia coli DF1020
-
containing overproducing plasmid pHE1007
-
Escherichia coli DF1020
-
containing overproducing plasmid pHE1012
-
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpressed PFK, DEAE-cellulose, Cibacron blue, hydroxyapatite Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate
-
Geobacillus stearothermophilus ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate
-
Escherichia coli DF1020 ADP + D-fructose 1,6-bisphosphate
-
?