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Literature summary for 2.7.1.11 extracted from

  • Kimmel, J.L.; Reinhart, G.D.
    Isolation of an individual allosteric interaction in tetrameric phosphofructokinase from Bacillus stearothermophilus (2001), Biochemistry, 40, 11623-11629.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
K90/91E surface charge-tag substitution, no change in Km for ATP and K1/2 for fructose 6-phosphate Geobacillus stearothermophilus
R162E single active site substitution, affinity for fructose 6-phosphate is diminished approx. 3 orders of magnitude relative to that of the wild-type Geobacillus stearothermophilus
R211E/K213E allosteric site substitutions Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
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