Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | activation | Oncorhynchus mykiss | |
D-fructose 2,6-bisphosphate | activation | Oncorhynchus mykiss |
General Stability | Organism |
---|---|
labile at all stages of purification, particularly at high dilutions or high salt concentrations, glycerol, fructose 6-phosphate and high enzyme concentrations stabilize | Oncorhynchus mykiss |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 0.99 mM, 50% inhibition | Oncorhynchus mykiss | |
citrate | 0.12 mM, 50% inhibition | Oncorhynchus mykiss | |
phosphoenolpyruvate | 0.59 mM, 50% inhibition | Oncorhynchus mykiss |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ATP | pH 7.2, 22°C | Oncorhynchus mykiss |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76500 | - |
6 * 76500, SDS-PAGE | Oncorhynchus mykiss |
478000 | - |
gel filtration | Oncorhynchus mykiss |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncorhynchus mykiss | - |
rainbow trout | - |
Purification (Comment) | Organism |
---|---|
Cellulose-P, hydroxyapatite, ATP-agarose | Oncorhynchus mykiss |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oncorhynchus mykiss | - |
skeletal muscle | white skeletal muscle | Oncorhynchus mykiss | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
76.5 | - |
- |
Oncorhynchus mykiss |
Storage Stability | Organism |
---|---|
-20°C, 0.06 mg protein/ml, in 20 mM phosphate buffer, pH 7.5, 40% v/v glycerol, 1 month | Oncorhynchus mykiss |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Oncorhynchus mykiss | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 76500, SDS-PAGE | Oncorhynchus mykiss |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Oncorhynchus mykiss |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | AMP, fructose 2,6-bisphosphate, and phosphate lower the pH optimum to approx. pH 7.6, 7.8 and 7.6, respectively | Oncorhynchus mykiss |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.5 | approx. half-maximal activity at pH 7.4, maximal activity at pH 8.5, activators shift the optimum to lower pH-values | Oncorhynchus mykiss |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oncorhynchus mykiss | isoelectric focusing | - |
7.1 |