EC Number   |
Protein Variants |
Reference |
|---|
    2.7.1.11 | D127/R252Q |
very low activity, unchanged Km for fructose 6-phosphate |
640519 |
| 2.7.1.11 | D127S |
almost no activity |
640519 |
| 2.7.1.11 | D543A |
the mutation causes an increased efficacy of ATP at the inhibitory allosteric binding site. The mutation drastically increases K0.5 for AMP. The activating effect of ADP found in wild type enzyme is completely lost |
721506 |
| 2.7.1.11 | D564N |
somatic mutation identified in human cancers. Mutant has decreased maximum velocity and affinity for fructose 6-phosphate |
739167 |
| 2.7.1.11 | E190Q |
the mutation drastically diminishes the kinetic affinity of this site for Mg2+ and Mn2+ |
721420 |
| 2.7.1.11 | E222A/H223A |
ability to be allosterically regulated is retained |
640517 |
| 2.7.1.11 | E657A |
mutant has reduced affinity of about 4.5 mM for frucotse 6-phosphate, compared with about 0.8 mM in wild type, and an 2fold decrease in maximum activity |
739167 |
| 2.7.1.11 | F76A |
62fold increase in Km for ATP |
640522 |
| 2.7.1.11 | F76W |
apparant Kms for ATP, ITP, GTP, CTP and UTP |
640522 |
| 2.7.1.11 | F76Y |
apparant Kms for ATP, ITP, GTP, CTP and UTP |
640522 |
