Literature summary for 2.7.1.11 extracted from

Byrnes, M.; Zhu, X.; Younathan, E.S.; Chang, S.H.
Kinetic characteristics of phosphofructokinase from Bacillus stearothermophilus: MgATP nonallosterically inhibits the enzyme (1994), Biochemistry, 33, 3424-3431.

Search for more literature for 2.7.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
G212V	inhibition by phosphoenolpyruvate can not be reversed by GDP or ADP, neither ADP nor GDP bind well to the effector site	Geobacillus stearothermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
MgATP2-	-	Geobacillus stearothermophilus
additional information	product inhibition of reverse reaction	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetic study	Geobacillus stearothermophilus
0.028	-	D-fructose 6-phosphate	pH 8.2, 30°C, cosubstrate GTP	Geobacillus stearothermophilus
0.03	-	D-fructose 6-phosphate	pH 8.2, 30°C, at fructose 6-phosphate concentrations that are less than approx. one-third the fixed MgATP concentration	Geobacillus stearothermophilus
0.068	-	D-fructose 6-phosphate	pH 8.2, 30°C, cosubstrate UTP	Geobacillus stearothermophilus
0.07	-	ATP	pH 8.2, 30°C	Geobacillus stearothermophilus
0.15	-	D-fructose 6-phosphate	pH 8.2, 30°C, cosubstrate CTP	Geobacillus stearothermophilus
0.18	-	GTP	pH 8.2, 30°C	Geobacillus stearothermophilus
2.6	-	CTP	pH 8.2, 30°C	Geobacillus stearothermophilus
2.8	-	UTP	pH 8.2, 30°C	Geobacillus stearothermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	expressed in PFK-deficient Escherichia coli DF1020 cells	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus stearothermophilus

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate	mechanism	Geobacillus stearothermophilus	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
160	-	-	Geobacillus stearothermophilus

Storage Stability

Storage Stability	Organism
-20°C, in 100 mM Tris-HCl buffer, pH 7.4, 1 mM dithiothreitol, 50% glycerol, stable	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose 6-phosphate	35fold lower reverse reaction velocity	Geobacillus stearothermophilus	ADP + D-fructose 1,6-bisphosphate	-	r
CTP + D-fructose 6-phosphate	-	Geobacillus stearothermophilus	CDP + D-fructose 1,6-bisphosphate	-	?
GTP + D-fructose 6-phosphate	-	Geobacillus stearothermophilus	GDP + D-fructose 1,6-bisphosphate	-	?
UTP + D-fructose 6-phosphate	-	Geobacillus stearothermophilus	UDP + D-fructose 1,6-bisphosphate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2	-	fructose 1,6-bisphosphate	reverse raction, recombinant enzyme	Geobacillus stearothermophilus
112	-	fructose 6-phosphate	forward reaction, recombinant enzyme	Geobacillus stearothermophilus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)