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Literature summary for 2.7.1.11 extracted from
- Purification, microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme (2007), FEBS J., 274, 429-438.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | slight inhibition; slight inhibition; slight inhibition | Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.081 | - |
ATP | - |
Spinacia oleracea | |
| 0.081 | - |
ATP | 30°C, pH 7.8 | Spinacia oleracea | |
| 1.7 | - |
D-fructose 6-phosphate | 30°C, pH 7.8 | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
4 * 52000, SDS-PAGE | Spinacia oleracea |
| 200000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
| Spinacia oleracea | A1XM06 | - |
- |
| Spinacia oleracea | A1XM07 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
| inclusion of adenosine 5'-(beta,gamma-imido)triphosphate is conducive to enzyme activitiy during the purification | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 600 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Spinacia oleracea | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 52000, SDS-PAGE | Spinacia oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-dependent phosphofructokinase | - |
Spinacia oleracea |
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Release 2023.1 (January 2023)
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