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Literature summary for 2.7.1.11 extracted from
- Phosphofructokinase from Bacillus licheniformis (1982), Methods Enzymol., 90, 70-77.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | - |
Bacillus licheniformis |  |
General Stability
| General Stability | Organism |
|---|---|
| D-fructose 6-phosphate stabilizes, less effective is KCl or phosphoenolpyruvate, only slightly stabilized by ATP or ATP/Mg2+ | Bacillus sp. (in: Bacteria) |
| D-fructose 6-phosphate stabilizes, less effective is KCl or phosphoenolpyruvate, only slightly stabilized by ATP or ATP/Mg2+ | Bacillus licheniformis |
| dilution inactivates | Bacillus sp. (in: Bacteria) |
| dilution inactivates | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | competitive vs. Mg2+ | Bacillus licheniformis | |
| citrate | - |
Bacillus licheniformis | |
| diphosphate | inactivates under assay conditions | Bacillus licheniformis | |
| phosphoenolpyruvate | effective inhibition at 0.005-0.02 mM, fructose 6-phosphate relieves inhibition; most potent inhibitor | Bacillus licheniformis | |
| phosphoenolpyruvate | most potent inhibitor | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
D-fructose 6-phosphate | - |
Bacillus sp. (in: Bacteria) | |
| 0.05 | - |
D-fructose 6-phosphate | pH 6.8, 30°C | Bacillus licheniformis | |
| 0.07 | - |
ATP | pH 6.8, 30°C | Bacillus licheniformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bacillus sp. (in: Bacteria) | 5829 | - |
| cytosol | - |
Bacillus licheniformis | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Bacillus sp. (in: Bacteria) | |
| K+ | - |
Bacillus licheniformis | |
| Mg2+ | required for activity | Bacillus sp. (in: Bacteria) | |
| Mg2+ | required for activity | Bacillus licheniformis | |
| additional information | not activated by Li+ | Bacillus sp. (in: Bacteria) | |
| additional information | not activated by Li+ | Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 135000 | - |
- |
Bacillus sp. (in: Bacteria) |
| 135000 | - |
sucrose density gradient centrifugation | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus sp. (in: Bacteria) | - |
e.g. Bacillus cereus, Bacillus megaterium, Bacillus mycoides, Bacillus subtilis 168M | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus sp. (in: Bacteria) |
| partial | Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
- |
Bacillus licheniformis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in phosphate buffer, 50% glycerol, 0.3% loss of activity per day | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Bacillus sp. (in: Bacteria) | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | - |
Bacillus licheniformis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bacillus sp. (in: Bacteria) |
| 30 | - |
assay at | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
- |
Bacillus sp. (in: Bacteria) |
- |
- |
rapid inactivation in the presence of Mg2+, phosphoenolpyruvate and ATP, incubation at 30°C reverses activity, inactivation is probably due to dissociation of a dimer into monomers | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | 8.7 | - |
Bacillus licheniformis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
Ca2+ | pH 6.8, 30°C | Bacillus licheniformis | |
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