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Literature summary for 2.7.1.11 extracted from

  • Kirchberger, J.; Bar, J.; Schellenberger, W.; Dihazi, H.; Kopperschlager, G.
    6-phosphofructokinase from Pichia pastoris: purification, kinetic and molecular characterization of the enzyme (2002), Yeast, 19, 933-947.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
AMP
-
 Komagataella pastoris
D-fructose 2,6-bisphosphate
-
 Komagataella pastoris

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Komagataella pastoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 113000 + x * 98000 + x * 34000, 34000 Da non-covalently complexed protein component seems to be necessary for activity, SDS-PAGE Komagataella pastoris
98000
-
 x * 113000 + x * 98000 + x * 34000, 34000 Da non-covalently complexed protein component seems to be necessary for activity, SDS-PAGE Komagataella pastoris
113000
-
 x * 113000 + x * 98000 + x * 34000, 34000 Da non-covalently complexed protein component seems to be necessary for activity, SDS-PAGE Komagataella pastoris
975000
-
 sedimentation equilibrium analysis Komagataella pastoris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Komagataella pastoris
-
 ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pseudo-affinity dye-ligand chromatography on Procion Blue H-5R-Sepharose Komagataella pastoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
-
 Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Komagataella pastoris ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate fructose 6-phosphate shows cooperativity Komagataella pastoris ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 113000 + x * 98000 + x * 34000, 34000 Da non-covalently complexed protein component seems to be necessary for activity, SDS-PAGE Komagataella pastoris