EC Number   |
Application |
Reference |
|---|
    2.7.1.11 | analysis |
assay for phosphofructokinase-1 using capillary electrophoresis based on the separation and detection by ultraviolet absorbance at 260 nm of Mg-ATP and Mg-ADP. The separation is enhanced by the addition of Mg2+ to the separation buffer. the assay for directly monitors the enzyme-catalyzed reaction |
737432 |
| 2.7.1.11 | biotechnology |
design of strains with improved antibiotic production |
693124 |
| 2.7.1.11 | medicine |
somatic PFK1 mutations identified in human cancers have distinct effects on allosteric regulation of enzymic activity and lactate production |
739167 |
| 2.7.1.11 | medicine |
total PFK1 levels are higher in human breast cancer tissues than in paracancer tissues. The human breast cancer and paracancer tissues mainly express PFK-P and PFK-L isoforms, respectively. Depending on the pathological stage of breast cancer, the expression of PFK1 is significantly positively correlated with the activity of PFK1 |
739209 |
| 2.7.1.11 | synthesis |
expression of a modified 6-phosphofructo-1-kinase in a citrate producing Aspergillus niger strain in combination with cis-aconitate decarboxylase CadA from Aspergillus terreus to study the effect on the production of itaconic acid. The combined expression of pfkA and cadA results in increased citrate levels, but does not show increased itaconic acid levels. The combined expression of pfkA with the itaconic acid biosynthetic cluster, consisiting of cis-aconitate decarboxylase cadA, a putative mitochondrial transporter mttA and a putative plasmamembrane transporter mfsA, results in significantly increased itaconic acid production at earlier time points and significant increase in itaconic acid productivity. The maximum itaconic acid productivity reached is 0.15 g per l and h |
737422 |
| 2.7.1.11 | synthesis |
expression of a truncated version of the gene encoding 6-phospho-1-fructokinase (tpfkA) along with its activator pkaC in Lactobacillus reuteri. Growth of the transformants at elevated glucose concentrations in the presence of fructose results in improved assimilation of the provided carbohydrates and a significant increase in the overall fermentation productivities. At the highest tested levels of glucose and fructose (75 g/l each), the transformant strain shows a 4fold increase in 6-phosphofructo-1-kinase activity and a 2.3fold increase in the glycolytic flux. The mannitol yield is 56 g/l compared to 10 g/l in the parental strain, and the lactate yield is 21 g/l (3.5 g/l in the parental strain). A high NADH/NAD+ratio occurrs under increased glycolytic flux conditions and facilitates the efficient conversion of fructose to mannitol |
-, 738734 |
| 2.7.1.11 | synthesis |
overexpression of both the 6-phosphofructokinase pfkA and pyruvate kinase pykA genes increase intracellular concentrations of ATP and NADH and also resistance to butanol toxicity. Marked increases of butanol and ethanol production, but not acetone, are observed in batch fermentation. The butanol and ethanol concentrations are 29.4 and 85.5% higher, respectively, in the fermentation by the double-overexpressing strain than the wild-type strain. In fed-batch fermentation using glucose, the butanol and total solvent (acetone, butanol, and ethanol) concentrations reach as high as 19.12 and 28.02 g/l, respectively |
737530 |
| 2.7.1.11 | synthesis |
sodium ions activated phosphofructokinase leads to enhanced D-lactic acid production by Sporolactobacillus inulinus using sodium hydroxide as a neutralizing agent |
-, 760404 |
