Literature summary for 2.7.1.11 extracted from

Harris, b.G.; Starling, J.A.; Hofer, H.W.
Phosphofructokinase from Ascaris suum muscle (1982), Methods Enzymol., 90, 44-49.

Search for more literature for 2.7.1.11

Activating Compound

Activating Compound	Comment	Organism	Structure
D-Glucose 1,6-bisphosphate	activation only in the presence of AMP	Ascaris suum
NH4+	-	Ascaris suum
SO42-	stimulation	Ascaris suum

Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	above 0.05 mM, inhibition is more pronounced at pH 6.6 but occurs also at pH 8.0, inhibition is reversed by equivalent amounts of AMP	Ascaris suum
citrate	cAMP, ADP or fructose 1,6-bisphosphate restore activity	Ascaris suum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	-	Ascaris suum
Mg2+	required for activity	Ascaris suum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
400000	-	-	Ascaris suum

Organism

Organism	UniProt	Comment	Textmining
Ascaris suum	-	female parasitic nematode, from small intestine of pig	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
side-chain modification	purified PFK contains 7-9 covalently bound phosphate groups per tetramer	Ascaris suum

Purification (Commentary)

Purification (Comment)	Organism
-	Ascaris suum

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Ascaris suum	-

Storage Stability

Storage Stability	Organism
4°C, in saturated ammonium sulfate solution, decrease of activity, dithiothreitol restores	Ascaris suum
4°C, under N2, in 50 mM Tris-potassium phosphate buffer, pH 8, 1 mM EDTA, 1.4 mM ammonium sulfate, 1 mM ATP, 0.2 mM KCl, 10 mM dithiothreitol, at least 6 months	Ascaris suum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose 6-phosphate	-	Ascaris suum	ADP + D-fructose 1,6-bisphosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 94600-95000, SDS-PAGE	Ascaris suum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Ascaris suum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)